http://2007.igem.org/wiki/index.php?title=UCSF/Microscopy&feed=atom&action=historyUCSF/Microscopy - Revision history2024-03-29T05:06:16ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=UCSF/Microscopy&diff=44250&oldid=prevSergio Peisajovich at 19:54, 26 October 20072007-10-26T19:54:57Z<p></p>
<p><b>New page</b></p><div>'''4- Microscopy'''<br />
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:Analysis of pathway-dependent Ste2 endocytosis by fluorescence microscopy was performed as follows: cultures were grown to early log phase (OD600=0.1-0.3) in complete synthetic dropout media and then transferred to a Concavaline-A treated 96-well fluorescence plate. Individual wells were observed at 100x in a Nikon Eclipse T2000 microscope. Ste2 endocytosis was induced by addition of 1 μM α-factor (Zymo Research) to activate the pathway. Time-lapse experiments were done by taking pictures every 1.5 minutes, for a total of up to 2 hours.</div>Sergio Peisajovich