UCSF/YeastStrains2

From 2007.igem.org

< UCSF(Difference between revisions)
 
Line 1: Line 1:
'''2- Yeast Strains'''
'''2- Yeast Strains'''
-
: 'Saccharomyces cerevisiae strains: (1) SP163 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-RR, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2), (2) SP164 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-HMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2),(3) SP165 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-WMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2).  
+
:Saccharomyces cerevisiae strains: (1) SP163 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-RR, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2), (2) SP164 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-HMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2),(3) SP165 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-WMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2).  
:Subsequently, each pRS315-based construct was transformed (selection based on Leu2 marker) into SP163.
:Subsequently, each pRS315-based construct was transformed (selection based on Leu2 marker) into SP163.

Latest revision as of 22:54, 24 October 2007

2- Yeast Strains

Saccharomyces cerevisiae strains: (1) SP163 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-RR, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2), (2) SP164 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-HMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2),(3) SP165 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-WMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2).
Subsequently, each pRS315-based construct was transformed (selection based on Leu2 marker) into SP163.