USTC/Demonstration

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An actual demonstration is determined to show the extensibility of our method.  
An actual demonstration is determined to show the extensibility of our method.  
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This demo system  
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• is designed as simple as possible, without any "cool" logic function;  
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• includes all the three logic gates, which constitute a three-level logic circuit;  
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This demo system
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• shows that wires can cross and branch off (without interference);  
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* is designed as simple as possible, without any "cool" logic function;  
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• is loaded on two plasmids, pSB1A3-I732998 and pSB1A3-I732999 ,and practically transformed into Top10 strain;  
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* includes all the three logic gates, which constitute a three-level logic circuit;  
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• accepts aTc and AHL signals as inputs;  
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* shows that wires can cross and branch off (without interference);  
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• produces RFP and GFP signals as outputs;  
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* is loaded on two plasmids, [[http://partsregistry.org/Part:BBa_I732923 pSB1A3-I732923]] and [[http://partsregistry.org/Part:BBa_I732925 pSB3K5-I732925]] ,and practically transformed into Top10 strain;  
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• can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in);  
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• is expected to put out the results that accord with the truth value shown in Figure 2.  
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* accepts aTc and AHL signals as inputs;  
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* produces RFP and GFP signals as outputs;  
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* can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in);  
 +
* is expected to put out the results that accord with the truth value shown in Figure 2.  
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In this truth table we can see that IPTG might result in all outputs lighten whether aTc or AHL exist. (It just likes the "8888..." on the screen when you reset your calculator or some other digital equipment.)
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We can see from this truth table that IPTG might result in both of the outputs being lightened no matter whether aTc or AHL exists or not. (It is just like the response "8888..." on the screen when you reset some of your electrical apparatus, for example, a calculator.)
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=Actual System=
=Actual System=
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The left three parts are loaded on [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998],
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The three parts on the left are loaded on [[http://partsregistry.org/Part:BBa_I732923 pSB1A3-I732923]], and the rest two parts are loaded on [[http://partsregistry.org/Part:BBa_I732925 pSB3K5-I732925]]. We've built up this system in one kind of TOP10 ''E.coli'', but it seems that it cannot grow stably when concentrated aTc exists. (We have to use aTc at a high concentration for counteracting the high expression of tetR, but concentrated aTc shows some bacteriostatic activity like Tc(TetraCycline).) We may not be able to send out the very final results here exactly by Oct. 26, but we have continually been trying our best to screen out such a monoclonal strain that is not influenced by concentrated aTc. You will get the results in the coming presentation...
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and the rest two parts are loaded on [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999]. We've built up this system in one kind of TOP10 ''E.coli'', but it seems that it cannot grow stably when aTc exist. (We have to use aTc at a high concentration since the high expression of LuxR.) I may not show the final results here before Oct. 26, but I'm trying my best to filter out a monoclonal strain for that. You will get the results in the coming presentation...
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Latest revision as of 04:33, 27 October 2007

An actual demonstration is determined to show the extensibility of our method.


This demo system

  • is designed as simple as possible, without any "cool" logic function;
  • includes all the three logic gates, which constitute a three-level logic circuit;
  • shows that wires can cross and branch off (without interference);
  • is loaded on two plasmids, [pSB1A3-I732923] and [pSB3K5-I732925] ,and practically transformed into Top10 strain;
  • accepts aTc and AHL signals as inputs;
  • produces RFP and GFP signals as outputs;
  • can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in);
  • is expected to put out the results that accord with the truth value shown in Figure 2.


Logic Abstract of the Demo

Figure 1 The logic diagram of the demo system.


Figure 2 The truth table of the demo system.


We can see from this truth table that IPTG might result in both of the outputs being lightened no matter whether aTc or AHL exists or not. (It is just like the response "8888..." on the screen when you reset some of your electrical apparatus, for example, a calculator.)

Actual System

Figure 3 The signal pathway of the demo system.


The three parts on the left are loaded on [pSB1A3-I732923], and the rest two parts are loaded on [pSB3K5-I732925]. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot grow stably when concentrated aTc exists. (We have to use aTc at a high concentration for counteracting the high expression of tetR, but concentrated aTc shows some bacteriostatic activity like Tc(TetraCycline).) We may not be able to send out the very final results here exactly by Oct. 26, but we have continually been trying our best to screen out such a monoclonal strain that is not influenced by concentrated aTc. You will get the results in the coming presentation...




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