http://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&feed=atom&action=historyUSTC/DualRepressedOperator - Revision history2024-03-29T11:49:08ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=48440&oldid=prevMaRui at 05:15, 27 October 20072007-10-27T05:15:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([https://2007.igem.org/USTC/Repressor_Evolution_on_Plates#Repression_Matrix more details about this Matrix])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([https://2007.igem.org/USTC/Repressor_Evolution_on_Plates#Repression_Matrix more details about this Matrix])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Remark: that best NOR above contains operator O11, which can be repressed by more than 3 kinds of repressors. It is just like the "<del class="diffchange diffchange-inline">wire </del>OR" in electronics, see also the <del class="diffchange diffchange-inline">Logic Abstract </del>in the right of Figure 1.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Remark: that best NOR above contains operator O11, which can be repressed by more than 3 kinds of repressors. It is just like the "<ins class="diffchange diffchange-inline">Wired </ins>OR" in electronics, see also the <ins class="diffchange diffchange-inline">logic abstract </ins>in the right of Figure 1.</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=48430&oldid=prevMaRui at 05:13, 27 October 20072007-10-27T05:13:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([https://2007.igem.org/USTC/Repressor_Evolution_on_Plates#Repression_Matrix more details about this Matrix])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([https://2007.igem.org/USTC/Repressor_Evolution_on_Plates#Repression_Matrix more details about this Matrix])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Remark: that best NOR above contains operator O11, which can be repressed by more than 3 kinds of repressors. It is just like the "wire OR" in electronics, <del class="diffchange diffchange-inline">refer to </del>the Logic Abstract in the right of Figure 1.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Remark: that best NOR above contains operator O11, which can be repressed by more than 3 kinds of repressors. It is just like the "wire OR" in electronics, <ins class="diffchange diffchange-inline">see also </ins>the Logic Abstract in the right of Figure 1.</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=48426&oldid=prevMaRui at 05:12, 27 October 20072007-10-27T05:12:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([https://2007.igem.org/USTC/Repressor_Evolution_on_Plates#Repression_Matrix more details about this Matrix])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([https://2007.igem.org/USTC/Repressor_Evolution_on_Plates#Repression_Matrix more details about this Matrix])</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Remark: that best NOR above contains operator O11, which can be repressed by more than 3 kinds of repressors. It is just like the "wire OR" in electronics, refer to the Logic Abstract in the right of Figure 1.</ins></div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=42791&oldid=prevMaRui at 16:22, 26 October 20072007-10-26T16:22:17Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([<del class="diffchange diffchange-inline">[</del>USTC/Repressor_Evolution_on_Plates<del class="diffchange diffchange-inline">|</del>more details about this Matrix<del class="diffchange diffchange-inline">]</del>])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two or more kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([<ins class="diffchange diffchange-inline">https://2007.igem.org/</ins>USTC/Repressor_Evolution_on_Plates<ins class="diffchange diffchange-inline">#Repression_Matrix </ins>more details about this Matrix])</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=42098&oldid=prevMaRui at 14:29, 26 October 20072007-10-26T14:29:05Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two kinds of repressors that can both bind on one kind of operator tightly<del class="diffchange diffchange-inline">.</del>. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates|more details about this Matrix]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, there may be two <ins class="diffchange diffchange-inline">or more </ins>kinds of repressors that can both bind on one kind of operator tightly. A promoter with this so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates|more details about this Matrix]])</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=42068&oldid=prevMaRui at 14:25, 26 October 20072007-10-26T14:25:16Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with <del class="diffchange diffchange-inline">a </del>so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates|more details about this Matrix]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, <ins class="diffchange diffchange-inline">there may be two kinds of repressors that can both bind on one kind of operator tightly.. A </ins>promoter with <ins class="diffchange diffchange-inline">this </ins>so-called "Dual-Repressed Operator" in the downstream can be used as a well-performing NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates|more details about this Matrix]])</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=41339&oldid=prevZhao Yun at 12:33, 26 October 20072007-10-26T12:33:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate <del class="diffchange diffchange-inline">that performs very well</del>. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates|more details about this Matrix]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a <ins class="diffchange diffchange-inline">well-performing </ins>NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates|more details about this Matrix]])</div></td></tr>
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</table>Zhao Yunhttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=41245&oldid=prevMaRui at 12:22, 26 October 20072007-10-26T12:22:29Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate that performs very well. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates more details about this Matrix]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate that performs very well. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). ([[USTC/Repressor_Evolution_on_Plates<ins class="diffchange diffchange-inline">|</ins>more details about this Matrix]])</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=41229&oldid=prevMaRui at 12:19, 26 October 20072007-10-26T12:19:32Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate that performs very well. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 and 2, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate that performs very well. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3). <ins class="diffchange diffchange-inline">([[USTC/Repressor_Evolution_on_Plates more details about this Matrix]])</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:USTC_RepressionMatrix.png|thumb|center|<del class="diffchange diffchange-inline">400px</del>|'''Figure 3''' Repression Matrix]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:USTC_RepressionMatrix.png|thumb|center|<ins class="diffchange diffchange-inline">450px</ins>|'''Figure 3''' Repression Matrix]]</div></td></tr>
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</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/DualRepressedOperator&diff=40499&oldid=prevMaRui at 10:04, 26 October 20072007-10-26T10:04:32Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| [[Image:USTC_DualRepressedOperator.png|thumb|<del class="diffchange diffchange-inline">300px</del>|'''Figure 1''' Sketch map of Dual-Repressed <del class="diffchange diffchange-inline">operator</del>.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| [[Image:USTC_DualRepressedOperator.png|thumb|<ins class="diffchange diffchange-inline">512px</ins>|'''Figure 1''' Sketch map of Dual-Repressed <ins class="diffchange diffchange-inline">Operator</ins>.]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| [[Image:USTC_BestNOR.png|thumb|<del class="diffchange diffchange-inline">200px</del>|'''Figure 2''' Best NOR, an example of Dual-Repressed <del class="diffchange diffchange-inline">operator</del>.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| [[Image:USTC_BestNOR.png|thumb|<ins class="diffchange diffchange-inline">256px</ins>|'''Figure 2''' Best NOR, an example of Dual-Repressed <ins class="diffchange diffchange-inline">Operators</ins>.]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As shown in Figure 1, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As shown in Figure 1 <ins class="diffchange diffchange-inline">and 2</ins>, promoter with a so-called "Dual-Repressed Operator" in the downstream can be used as a NOR gate <ins class="diffchange diffchange-inline">that performs very well</ins>. However, we faced a dilemma when integrating these dual-repressed NOR Gates into an actual system. It was because that most of the components in the system require wires exempt from interference while these NOR gates just in the contrary manner take the advantage of the mentioned interference. Therefore, the Dual-Repressed Operator should be carefully selected from the Repression Matrix (Figure 3).</div></td></tr>
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</table>MaRui