USTC/SuXiaofeng

From 2007.igem.org

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*;Work Process and Summary
*;Work Process and Summary
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#The Directed Evolution must be conducted on the base of 2 components-the repressor system and promoter-reporter system.By collaborations with other team members, I've successfully establish these two systems shown as below.
+
  1.The Directed Evolution must be conducted on the base of 2 components-the repressor system and promoter-reporter system.By collaborations with other team members, I've successfully establish these two systems shown as below.
-
#Within the work of establishment of two selection systems, a time consuming step is synthesis of target specific promoters that contain the mutated site design by Do. On account of economic and convenient aspect, I use 2-Step unparallel PCR by 3 fragments of primers and 2 times of PCR with different reaction conditions.As Below.
+
  2.Within the work of establishment of two selection systems, a time consuming step is synthesis of target specific promoters that contain the mutated site design by Do. On account of economic and convenient aspect, I use 2-Step unparallel PCR by 3 fragments of primers and 2 times of PCR with different reaction conditions.As Below.
*;Key Results
*;Key Results

Revision as of 13:37, 20 October 2007

Xiaofeng Su(Allen Su)-Undergraduate of Cellular and Molecular Biology in School of Life Sciences, USTC, P.R. China

XIAOFENG SU

Undergruduate Student of Cellular and Molecular Biology,USTC

Email: allensue@mail.ustc.edu.cn (preference) or xiaofsu@gmail.com

Phone: +86-551-3602469 (Lab)

Mobile: +86-13866722084

Address: Room 439, School of Life Sciences, USTC, Hefei, Anhui, P.R.China, 230026

Research Interest
  • Directed Evolution for Seeking New Protein-DNA Interactions
  • Approaches of Synthetic Biology for Forming Novel "Genetic Engineering Machines"
  • Differentiation and Development of Stem Cells

Research Work

  • Overall Description

For obtaining the signaling transduction parts of repression with high fidelity, I've experimentally designed and acquired some specific repressor-promoter pairs(R-P pairs or P-R pairs)based on Lactose Operon by directed evolution on plate. Besides, through quantitative assay,the novel artificial R-P pairs I selected have been tested for their binding performance so that P-R pairs of highest affinity and specificity can make a figure out of P-R pair candidates. Most of the parts in my work have been BioBricks-Standardized and work as BioBrick parts.

My project description by visual comics: All we need are the specific artificial 'repressor fish' that can definitely bite the specific 'operator hook' exclusively
My Work Basis-By Redesigning lac-repressor and operator. This figure comes from 'Roberto Kopke Salinas, etc. ChemBioChem 2005, 6, 1628 – 1637'
  • Experimental Design
  1. Construction of Expression Library of Lac-Repressor Family [Collaboration]
  2. Synthesis of Promoter Sequence with Specific Operators
  3. Construction of Low-copy Reporter System with Specific Opertors
  4. Selection of Promoter-Repressor Pair(P-R Pair) including re-testing validity of these combination
  5. Transfering the Operators to Double Reporter Systems [Collaboration]
  6. Quantitative assay of Repression Intensity and Specificity of P-R Pairs
  7. Results From RM(Repression Matrix) to ORM(Orthogonal RM)


  • Work Process and Summary
 1.The Directed Evolution must be conducted on the base of 2 components-the repressor system and promoter-reporter system.By collaborations with other team members, I've successfully establish these two systems shown as below.
 2.Within the work of establishment of two selection systems, a time consuming step is synthesis of target specific promoters that contain the mutated site design by Do. On account of economic and convenient aspect, I use 2-Step unparallel PCR by 3 fragments of primers and 2 times of PCR with different reaction conditions.As Below.
  • Key Results