USTC/ZhaoYun

From 2007.igem.org

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In detail, I have worked on four parts.
In detail, I have worked on four parts.
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The first one is that I designed to use PCR method to build up the procaryotic promoter family.  
The first one is that I designed to use PCR method to build up the procaryotic promoter family.  
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The second one is that I measured and compared different repression efficiency according to the different locations of the two operons.  
The second one is that I measured and compared different repression efficiency according to the different locations of the two operons.  
 +
The third is that I measured and compared different repression efficiency according to the different nucleotide sequence of the two operons, and have found a way to alter the combination intensity  of repressors.
The third is that I measured and compared different repression efficiency according to the different nucleotide sequence of the two operons, and have found a way to alter the combination intensity  of repressors.
 +
And the fourth one is that I discussed ranges of several parameters that are suitable for NAND, NOR, NOT Gate, with the help of data of DNA looping reported on previous papers. And I have attempted to synthesize and test the targeted artificial logic promoters.
And the fourth one is that I discussed ranges of several parameters that are suitable for NAND, NOR, NOT Gate, with the help of data of DNA looping reported on previous papers. And I have attempted to synthesize and test the targeted artificial logic promoters.
 +
Finally, I succeed to find the sequence pattern for NAND and NOT promoters, together with a not so perfect NOR promoter, in a systemetic method.
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 +
 +
More detailed process about promoter design and logic gate construction, please refer to  ----
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 +
Without doubt I must mention that all the work listed above is accomplished with much help of my senior fellow apprentices. I am really grateful for their help and kindness.
== Research Experience ==
== Research Experience ==

Revision as of 03:57, 26 October 2007

Ustc zhaoyun.jpg


Contents

Contact:

Address:

Room 438, Life Science Building, University of Science and Technology of China

Hefei, Anhui, P.R.China (230027)


Email:

zhaoyun@mail.ustc.edu.cn (preference)

or zhaoyunenator@gmail.com


Tel:

+86-551-3602469

Mobile:

+86-13866776861

Research Work

My major work in iGEM is to design and biologically implement three logic promoters: NAND,NOR and NOT. I attempt to systematically build up a procaryotic promoter family whose members contain different operons, that is, operons with different nucleotide sequences and different relative locations. We tested the expression activity under various combined signals of upstream repressors, and systematically study on how different operons influence the expression activity of repressors.

In detail, I have worked on four parts.

The first one is that I designed to use PCR method to build up the procaryotic promoter family.

The second one is that I measured and compared different repression efficiency according to the different locations of the two operons.

The third is that I measured and compared different repression efficiency according to the different nucleotide sequence of the two operons, and have found a way to alter the combination intensity of repressors.

And the fourth one is that I discussed ranges of several parameters that are suitable for NAND, NOR, NOT Gate, with the help of data of DNA looping reported on previous papers. And I have attempted to synthesize and test the targeted artificial logic promoters.

Finally, I succeed to find the sequence pattern for NAND and NOT promoters, together with a not so perfect NOR promoter, in a systemetic method.


More detailed process about promoter design and logic gate construction, please refer to ----

Without doubt I must mention that all the work listed above is accomplished with much help of my senior fellow apprentices. I am really grateful for their help and kindness.

Research Experience

(A)USTC iGEM Team Member Project: “Extensible Logic Circuit in Bacteria”. Succeed to find out the patterns for three bio-logic promoters, NAND, NOR, and NOT.

(B)Undergraduate Research Project Thesis title: “Artificial Bio-Logic Promoters, Model and Implementation”

(C)Undergraduate Internship Lab of Computational Biology, USTC Supervisor: Prof. Haiyan Liu

NNSFC(National Nature Science Foundation) Projects involved: “Theoretical Design and Experimental Analysis of Artificial Biological Network based on cell-cell communication”

Academic Activities

Presentation in Tianjin iGEM TTT Workshop Title: “Another Implementation of A Half Adder”