User:Meaganl/Online DNA submission
From 2007.igem.org
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iGEM 2007 Part Submission
In order to qualify for winning any part-related awards at the 2007 iGEM Jamboree, the teams must document their parts according to the Part Documentation Guidelines [PROVIDE LINK] as well as submit the physical DNA to the Registry. Following these guidelines will begin the part promotion process to get your part elevated to the Accepted rating level. Only parts that are at the Accepted rating level will be considered for awards.
For iGEM 2007 we are accepting part submissions in the form of miniprepped DNA. There is an online submission form that must be completed in order to start the DNA submission process (see more details below) [PROVIDE LINK TO FORM]. Each part will go through a physical DNA screening/quality control process, in addition to the part documentation review process, before it is accepted into the Registry. We will also provide online status updates for each part during the submission process so that the part creator can find out at what stage of the screening process their part has gone through.
Part Documentation Guidelines
Only parts that are deemed Accepted by the Registry will be considered for part-related awards in the iGEM 2007 Jamboree. In order to get your part promoted to the Accepted rating level, you must follow the instructions on the PART PROMOTION PAGE.
Quality Control Process
In order to assess the quality of the parts accepted into the Registry, we will perform a standard set of experiments with a focus on verifying the length of the insert. In addition we will aim to validate the declared antibiotic resistance of each part.
The quality control process is comprised of the following tasks:
- Transform: we will transform each sample of DNA into our standard cell strain, Top10
- Pick/Inoculate single colony: after transforming the DNA into Top10 cells, we will pick a single colony and inoculate into the antibiotic specified by the creator. We will also inoculate that colony into the rest of our standard antibiotic set (Ampicillin, Chloramphenicol, Tetracycline, Kanamycin) and watch for growth in the correct antibiotic
- Miniprep : the bacterial culture grown in the specified antibiotic will be miniprepped to isolate plasmid DNA
- Digest : the miniprepped plasmid DNA will be cut with EcoRI and PstI to cut out the insert from the plasmid
- Gel: the digested plasmid DNA will be run on an Invitrogen E-gel to visualize the insert and the plasmid. From this gel we can tell whether the part length corresponds to the length designated by the designer.
If the part length as detected on the E-gel corroborates what the designer has specified in the part documentation AND if the antibiotic testing is consistent with the expected antibiotic growth pattern, the physical DNA for the submitted part will be deemed Accepted.
