User:Silvapy
From 2007.igem.org
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[[Image:lainalab.jpg]] Pretending [[Image:grouplab.jpg]] No idea. ask Connie | [[Image:lainalab.jpg]] Pretending [[Image:grouplab.jpg]] No idea. ask Connie | ||
| - | My name is Laina | + | My name is Laina. |
| - | + | ||
| - | + | ||
This year I am on the UCB-LBNL IGEM team. | This year I am on the UCB-LBNL IGEM team. | ||
| - | My task this summer is to isolate and overexpress bacteriochlorophyll | + | |
| - | biosynthetic genes of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX. | + | |
| + | == Project == | ||
| + | |||
| + | My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX. | ||
| + | |||
| + | A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI abd BgII/XhoI site of the cosmid vector pET29bEBBX. | ||
== Project == | == Project == | ||
Revision as of 22:38, 22 October 2007
Contents |
Background
Pretending 
No idea. ask Connie
My name is Laina.
This year I am on the UCB-LBNL IGEM team.
Project
My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX.
A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI abd BgII/XhoI site of the cosmid vector pET29bEBBX.
Project
View My Presentation
Summer Project Diary
June Introductions
July
August
