User:Silvapy
From 2007.igem.org
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== Background == | == Background == | ||
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== Project == | == Project == | ||
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My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX. | My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX. | ||
A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI abd BgII/XhoI site of the cosmid vector pET29bEBBX. | A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI abd BgII/XhoI site of the cosmid vector pET29bEBBX. | ||
| - | + | '''Materials''' | |
| - | + | '''Methods''' | |
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| + | '''Results''' | ||
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| + | '''Discussion''' | ||
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| + | == Presentation == | ||
[[Image:IGEMPresentaion.jpg]] | [[Image:IGEMPresentaion.jpg]] | ||
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== Summer Project Diary == | == Summer Project Diary == | ||
June | June | ||
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August | August | ||
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Revision as of 22:40, 22 October 2007
Contents |
Background
Pretending 
No idea. ask Connie
My name is Laina.
This year I am on the UCB-LBNL IGEM team.
Project
Introduction My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX.
A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI abd BgII/XhoI site of the cosmid vector pET29bEBBX.
Materials
Methods
Results
Discussion
Presentation
Summer Project Diary
June
July
August
October
