Vaibhavi Umesh Notebook
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| + | __NOTOC__ | ||
| + | [[Template:BerkiGEM2007_VaibhaviConstructionFiles | My Construction Files]]<br> | ||
| + | [[Template:BerkiGEM2007_VaibhaviSequencingFiles | My Sequencing Files]]<br> | ||
| + | ---- | ||
| + | ==~~!~~== | ||
| + | notes | ||
| + | === === | ||
| + | <span style='font-family:"Courier New";color:#3366FF;font-size:6.0pt'> | ||
| + | to do | ||
| + | </span> | ||
==[[User:Vaibhaviu|Vaibhaviu]] 19:02, 1 June 2007 (EDT)== | ==[[User:Vaibhaviu|Vaibhaviu]] 19:02, 1 June 2007 (EDT)== | ||
Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:<br> | Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:<br> | ||
Revision as of 17:16, 3 June 2007
My Construction Files
My Sequencing Files
~~!~~
notes
to do
Vaibhaviu 19:02, 1 June 2007 (EDT)
Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:
It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file:
We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today.
