Valencia/Ligation

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Usually we ligate following the recommendations from the commercial information:
Usually we ligate following the recommendations from the commercial information:
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[[Image:lupa.jpg‎]] we have had good results with a molar ratio of 3:1
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[[Image:VLupa.jpg ]] we have had good results with a molar ratio of 3:1
*Ligase
*Ligase

Latest revision as of 06:46, 26 September 2007

Information obtained from:

Promega

We used one ligase for the ligations:

  • T4 DNA Ligase

Protocol obtained from: Promega


Ligation

Usually we ligate following the recommendations from the commercial information:

VLupa.jpg we have had good results with a molar ratio of 3:1

  • Ligase
total DNA 20-200 ng
enzyme 1 uL
2X Rapid Ligation buffer 10 uL
ddwater up to 20 uL (sometimes, if the samples are in low concentrations, we go up to 40uL)