Valencia/Ligation
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< Valencia(Difference between revisions)
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Usually we ligate following the recommendations from the commercial information: | Usually we ligate following the recommendations from the commercial information: | ||
- | [[Image: | + | [[Image:VLupa.jpg ]] we have had good results with a molar ratio of 3:1 |
*Ligase | *Ligase |
Latest revision as of 06:46, 26 September 2007
Information obtained from:
Promega
We used one ligase for the ligations:
- T4 DNA Ligase
Protocol obtained from: Promega
Ligation
Usually we ligate following the recommendations from the commercial information:
we have had good results with a molar ratio of 3:1
- Ligase
total DNA | 20-200 ng |
enzyme | 1 uL |
2X Rapid Ligation buffer | 10 uL |
ddwater | up to 20 uL (sometimes, if the samples are in low concentrations, we go up to 40uL) |