http://2007.igem.org/wiki/index.php?title=Valencia/Ligation&feed=atom&action=historyValencia/Ligation - Revision history2024-03-28T21:51:21ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Valencia/Ligation&diff=20956&oldid=prevEmilio at 06:46, 26 September 20072007-09-26T06:46:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Usually we ligate following the recommendations from the commercial information:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Usually we ligate following the recommendations from the commercial information:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:<del class="diffchange diffchange-inline">lupa</del>.<del class="diffchange diffchange-inline">jpg</del>]] we have had good results with a molar ratio of 3:1</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:<ins class="diffchange diffchange-inline">VLupa</ins>.<ins class="diffchange diffchange-inline">jpg </ins>]] we have had good results with a molar ratio of 3:1</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligase</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligase</div></td></tr>
</table>Emiliohttp://2007.igem.org/wiki/index.php?title=Valencia/Ligation&diff=20953&oldid=prevEmilio at 06:43, 26 September 20072007-09-26T06:43:44Z<p></p>
<p><b>New page</b></p><div>Information obtained from: <br />
<br />
''Promega''<br />
<br />
We used one ligase for the ligations:<br />
*T4 DNA Ligase<br />
Protocol obtained from: [http://www.promega.com/tbs/9pim180/9pim180.pdf Promega]<br />
<br />
----<br />
===Ligation===<br />
Usually we ligate following the recommendations from the commercial information:<br />
<br />
[[Image:lupa.jpg]] we have had good results with a molar ratio of 3:1<br />
<br />
*Ligase<br />
{|<br />
|total DNA<br />
| 20-200 ng<br />
|-<br />
|enzyme<br />
| 1 uL <br />
|-<br />
|2X Rapid Ligation buffer<br />
| 10 uL<br />
|-<br />
|ddwater<br />
| up to 20 uL (sometimes, if the samples are in low concentrations, we go up to 40uL)<br />
|}</div>Emilio