Vector choice

From 2007.igem.org

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Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb.
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Our final vector will be YCplac22,an YC-type (centromeric) shuttle vector.
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'''Vector Information''':
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'''Vector type''': plasmid
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'''Size (kb): ''' 4.854
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'''Construct size (kb): ''' 4.854
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'''Construction:''' pUC19
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'''Markers:''' TRP1, ampR
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'''Features:''' insert detection: lacZ'
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'''marker(s):''' TRP1, ampR
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'''promoter:''' lac
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'''replicon:''' ARS1, pMB1
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'''MCS:''' HindIII...EcoRI
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'''centromere:''' CEN4
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                     [[Image:YCplac22_map.jpg]]
                     [[Image:YCplac22_map.jpg]]
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Selected features                                                                 
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'''Selected features'''                                                                  
    
    
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lac_promoter         142 - 171
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lac_promoter                     142 - 171
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M13_pUC_rev_primer   185 - 207  
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-
M13_reverse_primer   206 - 224
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M13_pUC_rev_primer               185 - 207
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M13_forward20_primer 306 - 290
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M13_pUC_fwd_primer   321 - 299  
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M13_reverse_primer               206 - 224
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CEN4                 504 - 1356  
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-
Orf frame 2         2740 - 2066
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M13_forward20_primer             306 - 290
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TRP1                 2740 - 2066
+
 
-
AmpR_promoter       2983 - 3011
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M13_pUC_fwd_primer               321 - 299
-
Orf frame 2         3053 - 3913
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Ampicillin          3053 - 3913
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CEN4                             504 - 1356
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pBR322_origin        4068 - 4687 
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 +
Orf frame 2                       2740 - 2066
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 +
TRP1                             2740 - 2066
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 +
AmpR_promoter                     2983 - 3011
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 +
Orf frame 2                       3053 - 3913
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Unique restriction sites
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Ampicillin                        3053 - 3913
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HindIII  233
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PstI  249
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pBR322_origin                    4068 - 4687
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SalI  251
+
-
XbaI  257
+
-
BamHI  263
+
-
SmaI  270
+
-
KpnI  276
+
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SacI  282
+
-
EcoRI  284
+
-
NarI  446
+
-
SpeI  550
+
-
NheI  1801
+
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BglII  1986
+
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EcoRV  2455
+
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PmlI  2780
+
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AatII  2921
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Latest revision as of 11:36, 23 October 2007

Our final vector will be YCplac22,an YC-type (centromeric) shuttle vector.

Vector Information:

Vector type: plasmid

Size (kb): 4.854

Construct size (kb): 4.854

Construction: pUC19

Markers: TRP1, ampR

Features: insert detection: lacZ'

marker(s): TRP1, ampR

promoter: lac

replicon: ARS1, pMB1

MCS: HindIII...EcoRI

centromere: CEN4

                   YCplac22 map.jpg

Selected features

lac_promoter 142 - 171

M13_pUC_rev_primer 185 - 207

M13_reverse_primer 206 - 224

M13_forward20_primer 306 - 290

M13_pUC_fwd_primer 321 - 299

CEN4 504 - 1356

Orf frame 2 2740 - 2066

TRP1 2740 - 2066

AmpR_promoter 2983 - 3011

Orf frame 2 3053 - 3913

Ampicillin 3053 - 3913

pBR322_origin 4068 - 4687