Week 10
From 2007.igem.org
(Difference between revisions)
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- [http://partsregistry.org/Part:BBa_I763011 I763011]; | - [http://partsregistry.org/Part:BBa_I763011 I763011]; | ||
- | - [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part: | + | - [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007 I763007]); |
*Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026; | *Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026; |
Revision as of 20:46, 7 September 2007
- 09/03/07
- Miniprep for:
- Digestion for:
-I763005+I763015 with Spe/Pst1;
-B0015 with Eco/Xba;
- Band extraction;
- Ligation for:
- 09/04/07
- There are colonies for every ligations.
- Meeting.
- 09/05/07
Miniprep for:
- Digestion for:
- I763019 with Eco/Xba;
- I763026 with Eco/Spe;
- I763030 with Eco/Spe;
- I763011 with Xba/Pst1;
- R0051+ I13507(I763007) with Xba/Pst1;
- I763021 with Eco/Spe;
- Control digestion for I763019 with Eco/Pst1;
- Band extraction for
- I763019;
- I763026;
- I763011;
- Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
- Ligation O/N for:
-I763026+I763019(I763028);
-I763026+I763004(I763027);
- 09/06/07
Digestion for:
-I763019 with Eco/Xba;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe.
- Band extraction from electrophoresis gel for I763019 and I763026.
- Ligation for I763027 with new extracts.
- Transformations for the new ligations.
- 09/07/07