Week 10
From 2007.igem.org
Revision as of 09:14, 14 September 2007 by Guido Costa (Talk | contribs)
- 09/03/07
- Miniprep for:
- Digestion for:
-I763005+I763015 with Spe/Pst1;
-B0015 with Eco/Xba;
- Band extraction from gel for all digestion;
- Ligation for:
- 09/04/07
- There are colonies for every ligations.
- Meeting.
- 09/05/07
Miniprep for:
- Digestion for:
- I763019 with Eco/Xba;
- I763026 with Eco/Spe;
- I763030 with Eco/Spe;
- I763011 with Xba/Pst1;
- R0051+ I13507(I763007) with Xba/Pst1;
- I763021 with Eco/Spe;
- Control digestion for I763019 with Eco/Pst1;
- Band extraction from gel for:
- I763019;
- I763026;
- I763011;
- New control digestion for:
-I763019 with Xba/Spe;
-I763019 with Xba/Pst1;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe;
-I763030 with Eco/Spe;
- 09/06/07
Digestion for:
-I763019 with Eco/Xba;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe.
- Band extraction from electrophoresis gel for I763019 and I763026.
- Ligation for I763027 with new extracts.
- Transformations for the new ligations.
- 09/07/07
- Glycerol stocks preparation;
- We check without plasmid bacteria growth curve to individuate a model parameter.