Week 11

From 2007.igem.org

(Difference between revisions)
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-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;
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* '''Testing our devices'''
 
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We take 5 ml of LB culture ground in which we put a colony of bacteria which contain Ptet-LacI Plac-cI-LacY-GFP plasmid.
 
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We examine a drop of it using our fluorescence microscopy setup. In these conditions, the bacteria don’t beam fluorescence.
 
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Starting from this moment, we put into the culture ground 1 mM of IPTG every 30 minutes until it is 4 millimolar, checking each time any eventual fluorescence, with negative results.
 
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After diluting 1 ml of the previous one with 4 ml of LB, we add IPTG until 1 ml of our new solution is 10 mM, then 20 mM and finally 40 mM. Despite that, no fluorescence is visible.
 
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Every time we checked for fluorescence, we used not only the photo camera but also the photomultiplier; every measurement showed a 0.16-0.18 volts offset, despite the bacteria being non-fluorescent: at least, we regulated that offset!
 

Revision as of 11:14, 14 September 2007

09/10/07
  • Miniprep for

-I763027;

-I763028;

-I763019;

-I763021+P0412;

-I763021+S03520;

-I763021+S0100;

Digestion for:

-I763027 with Xba/Spe;

-I763028 with Spe/Pst1 and with Xba/Spe;

-I763019 with Xba/Spe;

-I763021+P0412 with Eco/Spe;

-I763021+S03520 with Eco/Spe;

-I763021+S0100 with Eco/Spe;

  • Band extraction from gel for all digestion;
  • We have problems with J52034 and with I763019.
  • Ligations for:

-I763028 + I763007

-S0100 + J04431, because we want to understand if LacI operates well;

-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.

09/11/07
  • Digestion for:

-I763020 with Xba/Pst1;

-J22101 with Xba/Pst1;


09/12/07



09/13/07



09/14/07



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