Week 11
From 2007.igem.org
(Difference between revisions)
Line 39: | Line 39: | ||
* Digestion for: | * Digestion for: | ||
-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1; | -[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1; | ||
+ | |||
-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1; | -[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1; | ||
Revision as of 10:38, 12 September 2007
- 09/10/07
- Miniprep for
-I763027;
-I763028;
-I763019;
Digestion for:
-I763027 with Xba/Spe;
-I763028 with Spe/Pst1 and with Xba/Spe;
-I763019 with Xba/Spe;
- Band extraction from gel for all digestion;
- We have problems with J52034 and with I763019.
- Ligations for:
-S0100 + J04431, because we want to understand if LacI operates well;
-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.
- 09/11/07
- Digestion for:
-I763020 with Xba/Pst1;
-J22101 with Xba/Pst1;
- 09/12/07
- 09/13/07
- 09/14/07