Week 11

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09/10/07
  • Miniprep for:

-I763027;

-I763028;

-I763019;

-I763021+P0412;

-I763021+S03520;

-I763021+S0100;

Digestion for:

-I763027 with Xba/Spe;

-I763028 with Spe/Pst1 and with Xba/Spe;

-I763019 with Xba/Spe;

-I763021+P0412 with Eco/Spe;

-I763021+S03520 with Eco/Spe;

-I763021+S0100 with Eco/Spe;

  • Band extraction from gel for all digestion;
  • We have problems with J52034 and with I763019.
  • Ligations for:

-I763028 + I763007

-S0100 + J04431, because we want to understand if LacI operates well;

-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.

09/11/07
  • Digestion for:

-I763020 with Xba/Pst1;

-J22101 with Xba/Pst1;


09/12/07
  • Control digestion before fluorescence tests. (photos)
  • Ligations for:

-I7633005(Spe/Pst1) + J04031(Xba/Pst1);

-S0100(Eco/Spe) + J04031(Eco/Xba);

-I763015 (Eco/Spa) + J04031(Eco/Xba).


09/13/07
  • We have find any colonies for I763015 + J04031;
  • Miniprep for:

- I7633005 + J04031;

-S0100 + J04031;

-I763028.

  • Control digestion for I763028.
  • M9 Medium and lactose stocks preparation.


09/14/07
  • Glicerol stocks for:

-I763028


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