Week 12

From 2007.igem.org

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*We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.
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Revision as of 13:06, 20 September 2007

09/17/07
  • Ligations for:

-I763026 + I763019;

-I763026 + I763004;

-I763025 + J04031.

  • We transform ligations and strake them on plates.


09/18/07
  • We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.


09/19/07
  • Miniprep for:

-I763027;

-J04431;

-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).

  • Digestion for:

-I763027 with Eco/Spe;

-J04431 with Eco/Spe;

-Ptet-LacI-GFP with Eco/Spe;

-I763007 with Eco/Xba;

-Plac-cI-LacY with Eco/Spe1;

  • Band extraction from gel for all digestion and then we observe:

-I763027, J04431 are died;

-Ptet-LacI-GFP is correct;

-I763007 not found;

-Plac-cI-LacY is correct.


09/20/07



09/21/07



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