Week 12
From 2007.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004]; | -[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004]; | ||
| - | -I763025 + J04031. | + | -[http://partsregistry.org/Part:BBa_I763025 I763025] + [http://partsregistry.org/Part:BBa_J04031 J04031]. |
*We transform ligations and strake them on plates. | *We transform ligations and strake them on plates. | ||
| Line 16: | Line 16: | ||
::'''09/19/07''' | ::'''09/19/07''' | ||
*Miniprep for: | *Miniprep for: | ||
| - | -I763027; | + | -[http://partsregistry.org/Part:BBa_I763027 I763027]; |
| - | -J04431; | + | -[http://partsregistry.org/Part:BBa_J04031 J04031]J04431; |
-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1). | -Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1). | ||
Revision as of 13:52, 20 September 2007
- 09/17/07
- Ligations for:
- We transform ligations and strake them on plates.
- 09/18/07
- We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.
- 09/19/07
- Miniprep for:
-I763027;
-J04031J04431;
-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).
- Digestion for:
-I763027 with Eco/Spe;
-J04431 with Eco/Spe;
-Ptet-LacI-GFP with Eco/Spe;
-I763007 with Eco/Xba;
-Plac-cI-LacY with Eco/Spe1;
- Band extraction from gel for all digestion and then we observe:
-I763027, J04431 are died;
-Ptet-LacI-GFP is correct;
-I763007 not found;
-Plac-cI-LacY is correct.
- 09/20/07
- 09/21/07
