Week 14

From 2007.igem.org

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::'''10/01/07'''
::'''10/01/07'''
 +
*Meeting: Ethical issue.
 +
*Work on image segmentation algorithm.
 +
::'''10/02/07'''
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*Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
*Run on electrophoresis gel.
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*[http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
 +
*Ligations for:
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::-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
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::-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].
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::'''10/02/07'''
 
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'''Experimental Setup'''
 
-
To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
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::'''10/03/07'''
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*Digestions of:
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
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*Run on electrophoresis gel.
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*[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
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*Transformations of:
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::-[http://partsregistry.org/Part:BBa_I763034 I763034];
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::-[http://partsregistry.org/Part:BBa_I763031 I763031];
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::-[http://partsregistry.org/Part:BBa_I763032 I763032].
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*Ligations for:
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::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
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::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
+
'''Experimental Setup'''
-
Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.  
+
*To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
 +
*In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
-
::'''10/03/07'''
+
*Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
-
 
+
::'''10/04/07'''
::'''10/04/07'''
 +
*Trasformation of:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
*We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).
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::'''10/05/07'''
::'''10/05/07'''
 +
*Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
 +
*Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
 +
*Control digestion of:
 +
::-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
 +
*Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.
-
 
+
[[Bologna#Diary | Back]]
-
[[Bologna | Back]]
+

Latest revision as of 15:51, 26 October 2007

10/01/07
  • Meeting: Ethical issue.
  • Work on image segmentation algorithm.


10/02/07
  • Digestion of pSB4A3;
  • Run on electrophoresis gel.
  • pSB4A3 and I763033 band extraction.
  • Ligations for:
-I763004+I763033;
-I763012+I763020(I763032).



10/03/07
  • Digestions of:
-I763032 with Eco/Pst1;
-I763031 with Eco/Pst1;
-I763036 with Eco/Pst1;
-pSB4A3 with Eco/Pst1.
-I763034;
-I763031;
-I763032.
  • Ligations for:
-I763031+pSB4A3;
-I763032+pSB4A3;

Experimental Setup

  • To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
  • In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
  • Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.


10/04/07
  • Trasformation of:
-I763031+pSB4A3;
-I763032+pSB4A3;



10/05/07
-I763034 with Xba/Pst1;
-I763031 with Eco/Xba and Xba/Pst1;
-I763032 with Eco/Xba and Xba/Pst1.
  • Run on electrophoresis gel and I763032 (Eco/Xba and Xba/Pst1) band extraction.


Back