Week 14
From 2007.igem.org
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::'''10/01/07''' | ::'''10/01/07''' | ||
| - | + | *Meeting: Ethical issue. | |
::'''10/02/07''' | ::'''10/02/07''' | ||
| - | + | *Digestion of PSB4A3; | |
| + | *Run on electrophoresis gel. | ||
| + | *pSB4A3 and Ptet-RFP band extraction. | ||
| + | *Ligation for | ||
| + | ::-pLac-GFP+I76303; | ||
| + | ::-I763012+I763020(I763032). | ||
::'''10/03/07''' | ::'''10/03/07''' | ||
| + | *Digestion of: | ||
| + | ::-pLac-Lacy-GFP with Eco/Pst1; | ||
| + | ::-I763031 with Eco/Pst1; | ||
| + | ::-I763036 with Eco/Pst1; | ||
| + | ::-pSB4A3 with Eco/Pst1. | ||
| + | *Run on electrophoresis gel. | ||
| + | *I763031, I763036 and pSB4A3 band extraction. | ||
'''Experimental Setup''' | '''Experimental Setup''' | ||
| - | To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency. | + | *To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency. |
| + | |||
| + | *In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier. | ||
| - | + | *Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings. | |
| - | |||
Revision as of 09:19, 8 October 2007
- 10/01/07
- Meeting: Ethical issue.
- 10/02/07
- Digestion of PSB4A3;
- Run on electrophoresis gel.
- pSB4A3 and Ptet-RFP band extraction.
- Ligation for
- -pLac-GFP+I76303;
- -I763012+I763020(I763032).
- 10/03/07
- Digestion of:
- -pLac-Lacy-GFP with Eco/Pst1;
- -I763031 with Eco/Pst1;
- -I763036 with Eco/Pst1;
- -pSB4A3 with Eco/Pst1.
- Run on electrophoresis gel.
- I763031, I763036 and pSB4A3 band extraction.
Experimental Setup
- To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
- In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
- Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
- 10/04/07
- 10/05/07
