Week 15
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
::'''10/08/07''' | ::'''10/08/07''' | ||
| - | + | *We inoculate: | |
| + | ::-I763031 (low copy plasmid); | ||
| + | ::-Plac-CI-Lacy (low copy plasmid); | ||
| + | ::-E.Coli; | ||
| + | ::-I763032 (high copy plasmid). | ||
| + | *Digestion of: | ||
| + | ::-I763035 with Eco/Pst1; | ||
| + | ::-I763007 with Eco/spe; | ||
| + | ::-Ptet-LacI-T with Eco/Xba; | ||
| + | ::-pSB4A3 with Spe/Xba. | ||
| + | *Ligation for: | ||
| + | ::-I763035+pSB4A3; | ||
| + | ::-I763007+Ptet-lacI-t; | ||
| + | ::-I763007+pSB4A3; | ||
| + | ::-I763032+pSB4A3. | ||
| + | *Run on electrophoresis gel. | ||
| + | *I763007 and Ptet-lacI-T band extraction. | ||
| + | *O/N ligation for: | ||
| + | ::-Ptet-LacI-T+I763007; | ||
| + | ::-Ptet-LacI+pSB4A3; | ||
| + | ::-I763032+pSB4A3; | ||
| + | ::-I763007+pSB4A3. | ||
Revision as of 08:07, 11 October 2007
- 10/08/07
- We inoculate:
- -I763031 (low copy plasmid);
- -Plac-CI-Lacy (low copy plasmid);
- -E.Coli;
- -I763032 (high copy plasmid).
- Digestion of:
- -I763035 with Eco/Pst1;
- -I763007 with Eco/spe;
- -Ptet-LacI-T with Eco/Xba;
- -pSB4A3 with Spe/Xba.
- Ligation for:
- -I763035+pSB4A3;
- -I763007+Ptet-lacI-t;
- -I763007+pSB4A3;
- -I763032+pSB4A3.
- Run on electrophoresis gel.
- I763007 and Ptet-lacI-T band extraction.
- O/N ligation for:
- -Ptet-LacI-T+I763007;
- -Ptet-LacI+pSB4A3;
- -I763032+pSB4A3;
- -I763007+pSB4A3.
- 10/09/07
- 10/10/07
- 10/11/07
- 10/12/07
