Week 15

From 2007.igem.org

(Difference between revisions)
Line 23: Line 23:
::-I763007+pSB4A3.
::-I763007+pSB4A3.
*We inoculate:
*We inoculate:
-
::-
+
::-I763031 (low copy plasmid);
 +
::-Plac-CI-Lacy (low copy plasmid);
 +
::-I763032 (high copy plasmid).
::'''10/09/07'''
::'''10/09/07'''
-
 
+
*Transformation of:
 +
::-pTet-LacI-T + I763007;
 +
::-pTet-LacI (low copy plasmid);

Revision as of 09:19, 11 October 2007

10/08/07
  • We inoculate:
-I763031 (low copy plasmid);
-Plac-CI-Lacy (low copy plasmid);
-E.Coli;
-I763032 (high copy plasmid).
  • Digestion of:
-I763035 with Eco/Pst1;
-I763007 with Eco/spe;
-Ptet-LacI-T with Eco/Xba;
-pSB4A3 with Spe/Xba.
  • Ligation for:
-I763035+pSB4A3;
-I763007+Ptet-lacI-t;
-I763007+pSB4A3;
-I763032+pSB4A3.
  • Run on electrophoresis gel.
  • I763007 and Ptet-lacI-T band extraction.
  • O/N ligation for:
-Ptet-LacI-T+I763007;
-Ptet-LacI+pSB4A3;
-I763032+pSB4A3;
-I763007+pSB4A3.
  • We inoculate:
-I763031 (low copy plasmid);
-Plac-CI-Lacy (low copy plasmid);
-I763032 (high copy plasmid).


10/09/07
  • Transformation of:
-pTet-LacI-T + I763007;
-pTet-LacI (low copy plasmid);


10/10/07



10/11/07



10/12/07



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