Week 15
From 2007.igem.org
(Difference between revisions)
Line 9: | Line 9: | ||
::-I763007 with Eco/spe; | ::-I763007 with Eco/spe; | ||
::-I763026 with Eco/Xba; | ::-I763026 with Eco/Xba; | ||
- | ::-pSB4A3 with Spe/Xba. | + | ::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba. |
*Ligation for: | *Ligation for: | ||
::-I763035+pSB4A3; | ::-I763035+pSB4A3; | ||
::-I763007+I763026; | ::-I763007+I763026; | ||
- | ::-I763007+pSB4A3; | + | ::-I763007+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
- | ::-I763032+pSB4A3. | + | ::-I763032+[http://partsregistry.org/Part:pSB4A3 pSB4A3]. |
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
*I763007 and I763026 band extraction. | *I763007 and I763026 band extraction. | ||
*O/N ligation for: | *O/N ligation for: | ||
::-I763026+I763007; | ::-I763026+I763007; | ||
- | ::-I763024+pSB4A3; | + | ::-I763024+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
- | ::-I763032+pSB4A3; | + | ::-I763032+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
- | ::-I763007+pSB4A3. | + | ::-I763007+[http://partsregistry.org/Part:pSB4A3 pSB4A3]. |
*We inoculate: | *We inoculate: | ||
::-I763031 (low copy plasmid); | ::-I763031 (low copy plasmid); | ||
Line 52: | Line 52: | ||
*Ligation for : | *Ligation for : | ||
::-I763016 (low copy plasmid)+GFP; | ::-I763016 (low copy plasmid)+GFP; | ||
- | ::-I763024+pSB4A3; | + | ::-I763024+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
::-I763024+C0012(high copy plasmid). | ::-I763024+C0012(high copy plasmid). | ||
*We inoculate in 6ml: | *We inoculate in 6ml: |
Revision as of 10:25, 12 October 2007
- 10/08/07
- We inoculate:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -E.Coli;
- -I763032 (high copy plasmid).
- Digestion of:
- -I763035 with Eco/Pst1;
- -I763007 with Eco/spe;
- -I763026 with Eco/Xba;
- -pSB4A3 with Spe/Xba.
- Ligation for:
- Run on electrophoresis gel.
- I763007 and I763026 band extraction.
- O/N ligation for:
- We inoculate:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763032 (high copy plasmid).
- 10/09/07
- Transformation of:
- -I763026 + I763007;
- -I763024 (low copy plasmid);
- -I763032 (low copy plasmid);
- -I763007 (low copy plasmid).
- We inoculate in 6ml:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid).
- Ligation for:
- -I763016 (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- -I763016 (low copy plasmid);
- -I763031(low copy plasmid).
- Digestion for :
- -I763031 with Xba/Pst1;
- -I763016 (low copy plasmid) with Spe/Pst1;
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- -I763016 (low copy plasmid)+GFP;
- -I763024+pSB4A3;
- -I763024+C0012(high copy plasmid).
- We inoculate in 6ml:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024(low copy plasmid).
- 10/11/07
- Stock preparation (500ul) of:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024(low copy plasmid).
- Miniprep of:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024(low copy plasmid).
- Fluorescence test of:
- -I763031 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid).
- Control digestion of:
- -I763031 with Xba/Pst1;
- -I763016 with Xba/Pst1 and Spe/Xba;
- -I763026 + I763007 with Xba/Pst1 and Spe/Xba;
- -I763024 with Xba/Pst1;
- -I763007 with Xba/Pst1.
- Run on electrophoresis gel and band extraction.
- Transformation of:
- -I763019;
- -I763024+C0012;
- -I763032.
- We inoculate
- -I763019.
- 10/12/07
- Miniprep of:
- -I763019.
- Digestion of:
- -I763019 with Xba/Pst1;
- -I763019 with Eco/Xba.
- Run on electrophoresis gel.