Week 15

From 2007.igem.org

(Difference between revisions)
Line 11: Line 11:
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
*Ligation for:
*Ligation for:
-
::-[http://partsregistry.org/Part:I763035 I763035]+pSB4A3;
+
::-[http://partsregistry.org/Part:I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
::-[http://partsregistry.org/Part:I763007 I763007]+I763026;
+
::-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:I763026 I763026];
::-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
::-[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
::-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
::-[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
*Run on electrophoresis gel.
*Run on electrophoresis gel.
-
*[http://partsregistry.org/Part:I763007 I763007] and I763026 band extraction.
+
*[http://partsregistry.org/Part:I763007 I763007] and [http://partsregistry.org/Part:I763026 I763026] band extraction.
*O/N ligation for:
*O/N ligation for:
::-[http://partsregistry.org/Part:I763026 I763026]+[http://partsregistry.org/Part:I763007 I763007];
::-[http://partsregistry.org/Part:I763026 I763026]+[http://partsregistry.org/Part:I763007 I763007];

Revision as of 10:43, 12 October 2007

10/08/07
  • We inoculate:
-I763031 (low copy plasmid);
-I763016(low copy plasmid);
-E.Coli;
-I763032 (high copy plasmid).
  • Digestion of:
-I763035 with Eco/Pst1;
-I763007 with Eco/spe;
-I763026 with Eco/Xba;
-pSB4A3 with Spe/Xba.
  • Ligation for:
-I763035+pSB4A3;
-I763007+I763026;
-I763007+pSB4A3;
-I763032+pSB4A3.
  • Run on electrophoresis gel.
  • I763007 and I763026 band extraction.
  • O/N ligation for:
-I763026+I763007;
-I763024+pSB4A3;
-I763032+pSB4A3;
-I763007+pSB4A3.
  • We inoculate:
-I763031(low copy plasmid);
-I763016(low copy plasmid);
-I763032 (high copy plasmid).


10/09/07
  • Transformation of:
-I763026 + I763007;
-I763024 (low copy plasmid);
-I763032 (low copy plasmid);
-I763007 (low copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid).
  • Ligation for:
-I763016 (Eco/Spe)+GFP.(Eco/Xba).


10/10/07
  • Miniprep of:
-I763016 (low copy plasmid);
-I763031(low copy plasmid).
  • Digestion for :
-I763031 with Xba/Pst1;
-I763016 (low copy plasmid) with Spe/Pst1;
  • Run on electrophoresis gel and I763016 band extraction.
  • Ligation for :
-I763016 (low copy plasmid)+GFP;
-I763024+pSB4A3;
-I763024+C0012(high copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024(low copy plasmid).
10/11/07
  • Stock preparation (500ul) of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024(low copy plasmid).
  • Miniprep of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026+ I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024(low copy plasmid).
  • Fluorescence test of:
-I763031 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid).
  • Control digestion of:
-I763031 with Xba/Pst1;
-I763016 with Xba/Pst1 and Spe/Xba;
-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
-I763024 with Xba/Pst1;
-I763007 with Xba/Pst1.
  • Run on electrophoresis gel and band extraction.
  • Transformation of:
-I763019;
-I763024+C0012C0012;
-I763032.
  • We inoculate
-I763019.
10/12/07
  • Miniprep of:
-I763019.
  • Digestion of:
-I763019 with Xba/Pst1;
-I763019 with Eco/Xba.
  • Run on electrophoresis gel.


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