Week 15

From 2007.igem.org

(Difference between revisions)
Line 49: Line 49:
::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid) with Spe/Pst1;
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid) with Spe/Pst1;
-
*Run on electrophoresis gel and I763016 band extraction.
+
*Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
*Ligation for :
*Ligation for :
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP;
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP;
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-
::-[http://partsregistry.org/Part:I763024 I763024]+C0012(high copy plasmid).
+
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:C0012 C0012](high copy plasmid).
*We inoculate in 6ml:
*We inoculate in 6ml:
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
Line 86: Line 86:
*Transformation of:
*Transformation of:
::-[http://partsregistry.org/Part:I763019 I763019];
::-[http://partsregistry.org/Part:I763019 I763019];
-
::-[http://partsregistry.org/Part:I763024 I763024]+C0012[http://partsregistry.org/Part:C0012 C0012];
+
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:C0012 C0012];
::-[http://partsregistry.org/Part:I763032 I763032].
::-[http://partsregistry.org/Part:I763032 I763032].
*We inoculate  
*We inoculate  

Revision as of 10:45, 12 October 2007

10/08/07
  • We inoculate:
-I763031 (low copy plasmid);
-I763016(low copy plasmid);
-E.Coli;
-I763032 (high copy plasmid).
  • Digestion of:
-I763035 with Eco/Pst1;
-I763007 with Eco/spe;
-I763026 with Eco/Xba;
-pSB4A3 with Spe/Xba.
  • Ligation for:
-I763035+pSB4A3;
-I763007+I763026;
-I763007+pSB4A3;
-I763032+pSB4A3.
  • Run on electrophoresis gel.
  • I763007 and I763026 band extraction.
  • O/N ligation for:
-I763026+I763007;
-I763024+pSB4A3;
-I763032+pSB4A3;
-I763007+pSB4A3.
  • We inoculate:
-I763031(low copy plasmid);
-I763016(low copy plasmid);
-I763032 (high copy plasmid).


10/09/07
  • Transformation of:
-I763026 + I763007;
-I763024 (low copy plasmid);
-I763032 (low copy plasmid);
-I763007 (low copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid).
  • Ligation for:
-I763016 (Eco/Spe)+GFP.(Eco/Xba).


10/10/07
  • Miniprep of:
-I763016 (low copy plasmid);
-I763031(low copy plasmid).
  • Digestion for :
-I763031 with Xba/Pst1;
-I763016 (low copy plasmid) with Spe/Pst1;
  • Run on electrophoresis gel and I763016 band extraction.
  • Ligation for :
-I763016 (low copy plasmid)+GFP;
-I763024+pSB4A3;
-I763024+C0012(high copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024(low copy plasmid).
10/11/07
  • Stock preparation (500ul) of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024(low copy plasmid).
  • Miniprep of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026+ I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024(low copy plasmid).
  • Fluorescence test of:
-I763031 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid).
  • Control digestion of:
-I763031 with Xba/Pst1;
-I763016 with Xba/Pst1 and Spe/Xba;
-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
-I763024 with Xba/Pst1;
-I763007 with Xba/Pst1.
  • Run on electrophoresis gel and band extraction.
  • Transformation of:
-I763019;
-I763024+C0012;
-I763032.
  • We inoculate
-I763019.
10/12/07
  • Miniprep of:
-I763019.
  • Digestion of:
-I763019 with Xba/Pst1;
-I763019 with Eco/Xba.
  • Run on electrophoresis gel.


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