Week 15
From 2007.igem.org
(Difference between revisions)
Line 45: | Line 45: | ||
*Miniprep of: | *Miniprep of: | ||
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); | ||
- | ::-[http://partsregistry.org/Part:I763031 I763031](low copy plasmid). | + | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid). |
*Digestion for : | *Digestion for : | ||
::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1; | ::-[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1; | ||
Line 53: | Line 53: | ||
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP; | ::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP; | ||
::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3]; | ||
- | ::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:C0012 C0012](high copy plasmid). | + | ::-[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:C0012 C0012] (high copy plasmid). |
*We inoculate in 6ml: | *We inoculate in 6ml: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
Line 59: | Line 59: | ||
::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | ||
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ||
- | ::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | + | ::-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid). |
::'''10/11/07''' | ::'''10/11/07''' | ||
*Stock preparation (500ul) of: | *Stock preparation (500ul) of: | ||
Line 66: | Line 66: | ||
::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | ||
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ||
- | ::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | + | ::-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid). |
*Miniprep of: | *Miniprep of: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
Line 72: | Line 72: | ||
::-[http://partsregistry.org/Part:I763026 I763026]+ [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | ::-[http://partsregistry.org/Part:I763026 I763026]+ [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | ||
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid); | ||
- | ::-[http://partsregistry.org/Part:I763024 I763024](low copy plasmid). | + | ::-[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid). |
*Fluorescence test of: | *Fluorescence test of: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
- | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid); | + | ::-[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007] (high copy plasmid); |
::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid). | ::-[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid). | ||
*Control digestion of: | *Control digestion of: |
Revision as of 10:46, 12 October 2007
- 10/08/07
- We inoculate:
- Digestion of:
- Ligation for:
- We inoculate:
- 10/09/07
- Transformation of:
- We inoculate in 6ml:
- Ligation for:
- -I763016 (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- Digestion for :
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- We inoculate in 6ml:
- Stock preparation (500ul) of:
- Miniprep of:
- Fluorescence test of:
- Control digestion of:
- Run on electrophoresis gel and band extraction.
- Transformation of:
- We inoculate
- -I763019.
- 10/12/07
- Miniprep of:
- -I763019.
- Digestion of:
- Run on electrophoresis gel.