Week 15
From 2007.igem.org
(Difference between revisions)
Line 97: | Line 97: | ||
::-[http://partsregistry.org/Part:I763019 I763019] with Eco/Xba. | ::-[http://partsregistry.org/Part:I763019 I763019] with Eco/Xba. | ||
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
+ | *[http://partsregistry.org/Part:I763019 I763019] (Xba/Pst1) and [http://partsregistry.org/Part:I763019 I763019] (Eco/Xba) band extraction. | ||
[[Bologna | Back]] | [[Bologna | Back]] |
Revision as of 12:39, 12 October 2007
- 10/08/07
- We inoculate:
- Digestion of:
- Ligation for:
- We inoculate:
- 10/09/07
- Transformation of:
- We inoculate in 6ml:
- Ligation for:
- -I763016 (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- Digestion for :
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- We inoculate in 6ml:
- Stock preparation (500ul) of:
- Miniprep of:
- Fluorescence test of:
- Control digestion of:
- Run on electrophoresis gel and band extraction.
- Transformation of:
- We inoculate
- -I763019.
- 10/12/07
- Miniprep of:
- -I763019.
- Digestion of: