Week 15

From 2007.igem.org

(Difference between revisions)
Line 2: Line 2:
*We inoculate:
*We inoculate:
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-
::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
+
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
::-E.Coli;
::-E.Coli;
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).

Revision as of 09:36, 24 October 2007

10/08/07
  • We inoculate:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-E.Coli;
-I763032 (high copy plasmid).
  • Digestion of:
-I763035 with Eco/Pst1;
-I763007 with Eco/spe;
-I763026 with Eco/Xba;
-pSB4A3 with Spe/Xba.
  • Ligation for:
-I763035+pSB4A3;
-I763007+I763026;
-I763007+pSB4A3;
-I763032+pSB4A3.
  • Run on electrophoresis gel.
  • I763007 and I763026 band extraction.
  • O/N ligation for:
-I763026+I763007;
-I763024+pSB4A3;
-I763032+pSB4A3;
-I763007+pSB4A3.
  • We inoculate:
-I763031(low copy plasmid);
-I763016(low copy plasmid);
-I763032 (high copy plasmid).


10/09/07
  • Transformation of:
-I763026 + I763007;
-I763024 (low copy plasmid);
-I763032 (low copy plasmid);
-I763007 (low copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid).
  • Ligation for:
-I763016 (Eco/Spe)+GFP.(Eco/Xba).


10/10/07
  • Miniprep of:
-I763016 (low copy plasmid);
-I763031 (low copy plasmid).
  • Digestion for :
-I763031 with Xba/Pst1;
-I763016 (low copy plasmid) with Spe/Pst1;
  • Run on electrophoresis gel and I763016 band extraction.
  • Ligation for :
-I763016 (low copy plasmid)+GFP;
-I763024+pSB4A3;
-I763024+C0012 (high copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024 (low copy plasmid).
10/11/07
  • Stock preparation (500ul) of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024 (low copy plasmid).
  • Miniprep of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026+ I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024 (low copy plasmid).
  • Fluorescence test of:
-I763031 (low copy plasmid);
-I763026 + I763007 (high copy plasmid);
-I763007 (low copy plasmid).
  • Control digestion of:
-I763031 with Xba/Pst1;
-I763016 with Xba/Pst1 and Spe/Xba;
-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
-I763024 with Xba/Pst1;
-I763007 with Xba/Pst1.
  • Run on electrophoresis gel and band extraction.
  • Transformation of:
-I763019;
-I763024+C0012;
-I763032.
  • We inoculate
-I763019.
10/12/07
  • Miniprep of:
-I763019.
  • Digestion of:
-I763019 with Xba/Pst1;
-I763019 with Eco/Xba.
  • Run on electrophoresis gel.
  • I763019 (Xba/Pst1) and I763019 (Eco/Xba) band extraction.


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