Week 15

From 2007.igem.org

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::'''10/01/07'''
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::'''10/08/07'''
 +
*We inoculate:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-E.Coli;
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).
 +
*Digestion of:
 +
::-[http://partsregistry.org/Part:BBa_I763035 I763035] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/spe;
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Xba;
 +
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
 +
*Ligation for:
 +
::-[http://partsregistry.org/Part:BBa_I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:BBa_I763026 I763026];
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
 +
*Run on electrophoresis gel.
 +
*[http://partsregistry.org/Part:BBa_I763007 I763007] and [http://partsregistry.org/Part:BBa_I763026 I763026] band extraction.
 +
*O/N ligation for:
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026]+[http://partsregistry.org/Part:BBa_I763007 I763007];
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
 +
*We inoculate:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031](low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016](low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).
 +
::'''10/09/07'''
 +
*Transformation of:
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007];
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).
 +
*We inoculate in 6ml:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid).
 +
*Ligation for:
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).
-
::'''10/02/07'''
 
 +
::'''10/10/07'''
 +
*Miniprep of:
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid).
 +
*Digestion for :
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid) with Spe/Pst1;
 +
*Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
 +
*Ligation for :
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid)+GFP;
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012] (high copy plasmid).
 +
*We inoculate in 6ml:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
 +
::'''10/11/07'''
 +
*Stock preparation (500ul) of:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
 +
*Miniprep of:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
 +
*Fluorescence test of:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] (high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).
 +
*Control digestion of:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] with Xba/Pst1 and Spe/Xba;
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1 and Spe/Xba;
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024] with Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1.
 +
*Run on electrophoresis gel and band extraction.
 +
*Transformation of:
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019];
 +
::-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032].
 +
*We inoculate
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019].   
 +
::'''10/12/07'''
 +
*Miniprep of:
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019].
 +
*Digestion of:
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba.
 +
*Run on electrophoresis gel.
 +
*[http://partsregistry.org/Part:BBa_I763019 I763019] (Xba/Pst1) and [http://partsregistry.org/Part:BBa_I763019 I763019] (Eco/Xba) band extraction.
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::'''10/03/07'''
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[[Bologna#Diary | Back]]
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::'''10/04/07'''
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::'''10/05/07'''
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[[Bologna | Back]]
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Latest revision as of 15:51, 26 October 2007

10/08/07
  • We inoculate:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-E.Coli;
-I763032 (high copy plasmid).
  • Digestion of:
-I763035 with Eco/Pst1;
-I763007 with Eco/spe;
-I763026 with Eco/Xba;
-pSB4A3 with Spe/Xba.
  • Ligation for:
-I763035+pSB4A3;
-I763007+I763026;
-I763007+pSB4A3;
-I763032+pSB4A3.
  • Run on electrophoresis gel.
  • I763007 and I763026 band extraction.
  • O/N ligation for:
-I763026+I763007;
-I763024+pSB4A3;
-I763032+pSB4A3;
-I763007+pSB4A3.
  • We inoculate:
-I763031(low copy plasmid);
-I763016(low copy plasmid);
-I763032 (high copy plasmid).


10/09/07
  • Transformation of:
-I763026 + I763007;
-I763024 (low copy plasmid);
-I763032 (low copy plasmid);
-I763007 (low copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid).
  • Ligation for:
-I763016 (Eco/Spe)+GFP.(Eco/Xba).


10/10/07
  • Miniprep of:
-I763016 (low copy plasmid);
-I763031 (low copy plasmid).
  • Digestion for :
-I763031 with Xba/Pst1;
-I763016 (low copy plasmid) with Spe/Pst1;
  • Run on electrophoresis gel and I763016 band extraction.
  • Ligation for :
-I763016 (low copy plasmid)+GFP;
-I763024+pSB4A3;
-I763024+C0012 (high copy plasmid).
  • We inoculate in 6ml:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024 (low copy plasmid).
10/11/07
  • Stock preparation (500ul) of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026 + I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024 (low copy plasmid).
  • Miniprep of:
-I763031 (low copy plasmid);
-I763016 (low copy plasmid);
-I763026+ I763007(high copy plasmid);
-I763007 (low copy plasmid);
-I763024 (low copy plasmid).
  • Fluorescence test of:
-I763031 (low copy plasmid);
-I763026 + I763007 (high copy plasmid);
-I763007 (low copy plasmid).
  • Control digestion of:
-I763031 with Xba/Pst1;
-I763016 with Xba/Pst1 and Spe/Xba;
-I763026 + I763007 with Xba/Pst1 and Spe/Xba;
-I763024 with Xba/Pst1;
-I763007 with Xba/Pst1.
  • Run on electrophoresis gel and band extraction.
  • Transformation of:
-I763019;
-I763024+C0012;
-I763032.
  • We inoculate
-I763019.
10/12/07
  • Miniprep of:
-I763019.
  • Digestion of:
-I763019 with Xba/Pst1;
-I763019 with Eco/Xba.
  • Run on electrophoresis gel.
  • I763019 (Xba/Pst1) and I763019 (Eco/Xba) band extraction.


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