Week 15
From 2007.igem.org
(Difference between revisions)
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*We inoculate: | *We inoculate: | ||
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid); | ||
- | ::-[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid); |
::-E.Coli; | ::-E.Coli; | ||
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid). | ::-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid). | ||
Line 101: | Line 101: | ||
- | [[Bologna | Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:51, 26 October 2007
- 10/08/07
- We inoculate:
- Digestion of:
- Ligation for:
- We inoculate:
- 10/09/07
- Transformation of:
- We inoculate in 6ml:
- Ligation for:
- -I763016 (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- Digestion for :
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- We inoculate in 6ml:
- Stock preparation (500ul) of:
- Miniprep of:
- Fluorescence test of:
- Control digestion of:
- Run on electrophoresis gel and band extraction.
- Transformation of:
- We inoculate
- -I763019.
- 10/12/07
- Miniprep of:
- -I763019.
- Digestion of: