From 2007.igem.org
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- | [[Bologna | Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:51, 26 October 2007
- 10/08/07
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -E.Coli;
- -I763032 (high copy plasmid).
- -I763035 with Eco/Pst1;
- -I763007 with Eco/spe;
- -I763026 with Eco/Xba;
- -pSB4A3 with Spe/Xba.
- -I763035+pSB4A3;
- -I763007+I763026;
- -I763007+pSB4A3;
- -I763032+pSB4A3.
- Run on electrophoresis gel.
- I763007 and I763026 band extraction.
- O/N ligation for:
- -I763026+I763007;
- -I763024+pSB4A3;
- -I763032+pSB4A3;
- -I763007+pSB4A3.
- -I763031(low copy plasmid);
- -I763016(low copy plasmid);
- -I763032 (high copy plasmid).
- 10/09/07
- -I763026 + I763007;
- -I763024 (low copy plasmid);
- -I763032 (low copy plasmid);
- -I763007 (low copy plasmid).
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid).
- -I763016 (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- -I763016 (low copy plasmid);
- -I763031 (low copy plasmid).
- -I763031 with Xba/Pst1;
- -I763016 (low copy plasmid) with Spe/Pst1;
- Run on electrophoresis gel and I763016 band extraction.
- Ligation for :
- -I763016 (low copy plasmid)+GFP;
- -I763024+pSB4A3;
- -I763024+C0012 (high copy plasmid).
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024 (low copy plasmid).
- 10/11/07
- Stock preparation (500ul) of:
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026 + I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024 (low copy plasmid).
- -I763031 (low copy plasmid);
- -I763016 (low copy plasmid);
- -I763026+ I763007(high copy plasmid);
- -I763007 (low copy plasmid);
- -I763024 (low copy plasmid).
- -I763031 (low copy plasmid);
- -I763026 + I763007 (high copy plasmid);
- -I763007 (low copy plasmid).
- -I763031 with Xba/Pst1;
- -I763016 with Xba/Pst1 and Spe/Xba;
- -I763026 + I763007 with Xba/Pst1 and Spe/Xba;
- -I763024 with Xba/Pst1;
- -I763007 with Xba/Pst1.
- Run on electrophoresis gel and band extraction.
- Transformation of:
- -I763019;
- -I763024+C0012;
- -I763032.
- -I763019.
- 10/12/07
- -I763019.
- -I763019 with Xba/Pst1;
- -I763019 with Eco/Xba.
- Run on electrophoresis gel.
- I763019 (Xba/Pst1) and I763019 (Eco/Xba) band extraction.
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