Week 16
From 2007.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
::'''10/16/07''' | ::'''10/16/07''' | ||
| - | + | *Transformation of Plac-CI-LacY-GFP and pTet-LacI-T-Plambda-RFP. | |
| + | *We inoculate: | ||
| + | ::-Plac-CI; | ||
| + | ::-RBS-LacY; | ||
| + | ::-Plac-GFP. | ||
::'''10/17/07''' | ::'''10/17/07''' | ||
| - | + | *Miniprep of: | |
| - | + | ::-Plac-CI; | |
| + | ::-RBS-LacY; | ||
| + | ::-Plac-GFP. | ||
| + | *We inoculate: | ||
| + | ::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp; | ||
| + | ::-pTet-LacI-T-pLambda-rfp (high copy). | ||
| + | éDigestion of | ||
Revision as of 07:42, 18 October 2007
- 10/15/07
- O/N ligations for:
- -Plac-CI-LacY-GFP;
- -pTet-LacI-T-Plambda-RFP.
- Run on electrophoresis gel and Plac-CI-LacY-GFP band extraction.
- 10/16/07
- Transformation of Plac-CI-LacY-GFP and pTet-LacI-T-Plambda-RFP.
- We inoculate:
- -Plac-CI;
- -RBS-LacY;
- -Plac-GFP.
- 10/17/07
- Miniprep of:
- -Plac-CI;
- -RBS-LacY;
- -Plac-GFP.
- We inoculate:
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;
- -pTet-LacI-T-pLambda-rfp (high copy).
éDigestion of
- 10/18/07
- 10/19/07
