Week 16

From 2007.igem.org

(Difference between revisions)
Line 72: Line 72:
      
      
::'''10/19/07'''
::'''10/19/07'''
 +
*Transformation:
 +
::-pLac-GFP-pTet-LacI (on high copy plasmid);::
 +
::-pTet-LacI-T-Plambda-RFP;
 +
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).

Revision as of 08:46, 23 October 2007

10/15/07
  • O/N ligations for:
-I763019;
-pTet-LacI-T-Plambda-RFP.



10/16/07
  • Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
  • We inoculate:
-I763005;
-I763015;
-I763004.
  • Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.


10/17/07
  • Miniprep of:
-I763005;
-I763015;
-I763004.
  • We inoculate:
-Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;
-pTet-LacI-T-pLambda-rfp (high copy).
  • Control Digestion for results impagination (X/P):
- I763020;
- I763004;
- I763011;
- I763007 (on high copy plasmid);
- I763007 (on low copy plasmid);
- I763033;
- I763015;
- I763012;
- I763005;
- I763031.


10/18/07
  • Miniprep:
-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;
-pTet-LacI-T-pLambda-rfp (high copy).
  • Digestion for:
-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
  • Band extraction of:
-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
  • Ligation for:
-pSB4A3 (E/P)
-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
  • Ligation for:
-pLac-GFP(X/P)

- pTetR-LacI (S/P)

  • Control Digestion for results impagination:
- I763016 (low copy plasmid);
- I763016 (high copy plasmid);
- I763036;
- I763019;
- I763032;
- I763025 (low copy plasmid);
- I763025 (high copy plasmid);
- I763026;
- I763035.



10/19/07
  • Transformation:
-pLac-GFP-pTet-LacI (on high copy plasmid);::
-pTet-LacI-T-Plambda-RFP;
-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).



Back