Week 16
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
::'''10/15/07''' | ::'''10/15/07''' | ||
*O/N ligations for: | *O/N ligations for: | ||
| - | ::-I763019; | + | ::-[http://partsregistry.org/Part:BBa_I763019 I763019]; |
| - | ::-I763039. | + | ::-[http://partsregistry.org/Part:BBa_I763039 I763039]. |
| Line 8: | Line 8: | ||
::'''10/16/07''' | ::'''10/16/07''' | ||
| - | *Transformation of I763019 and I763039. | + | *Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and I763039. |
*We inoculate: | *We inoculate: | ||
::-I763005; | ::-I763005; | ||
| Line 61: | Line 61: | ||
::- I763016 (high copy plasmid); | ::- I763016 (high copy plasmid); | ||
::- I763036; | ::- I763036; | ||
| - | ::- I763019; | + | ::- [http://partsregistry.org/Part:BBa_I763019 I763019]; |
::- I763032; | ::- I763032; | ||
::- I763025 (low copy plasmid); | ::- I763025 (low copy plasmid); | ||
Revision as of 09:09, 23 October 2007
- 10/15/07
- O/N ligations for:
- 10/16/07
- Transformation of I763019 and I763039.
- We inoculate:
- -I763005;
- -I763015;
- -I763004.
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- -I763005;
- -I763015;
- -I763004.
- We inoculate:
- -I763029;
- -I763039 (high copy).
- Control Digestion for results impagination (X/P):
- - I763020;
- - I763004;
- - I763011;
- - I763007 (on high copy plasmid);
- - I763007 (on low copy plasmid);
- - I763033;
- - I763015;
- - I763012;
- - I763005;
- - I763031.
- 10/18/07
- Miniprep:
- -I763029;
- -I763039(high copy).
- Digestion for:
- -I763029 (E/P)
- Band extraction of:
- -I763029 (E/P)
- Ligation for:
- -pSB4A3 (E/P)
- -I763029 (E/P)
- Ligation for:
- -I763004 (X/P)
- -I763026 (S/P)
- Control Digestion for results impagination:
- - I763016 (low copy plasmid);
- - I763016 (high copy plasmid);
- - I763036;
- - I763019;
- - I763032;
- - I763025 (low copy plasmid);
- - I763025 (high copy plasmid);
- - I763026;
- - I763035.
- 10/19/07
- Transformation:
- -I763040(on high copy plasmid);::
- -I763039;
- -I763029 on pSB4A3 (low copy plasmid).
