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	::'''10/19/07'''		::'''10/19/07'''
	*Transformation:		*Transformation:
-	::-I763040(on high copy plasmid);::	+	::-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);::
-	::-I763039;	+	::-[http://partsregistry.org/Part:BBa_I763039 I763039];
-	::-I763029 on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).

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Revision as of 09:20, 23 October 2007

10/15/07

• O/N ligations for:

-I763019;

-I763039.

10/16/07

• Transformation of I763019 and I763039.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-I763029;

-I763039 (high copy).

• Control Digestion for results impagination (X/P):

- I763020;

- I763004;

- I763011;

- I763007 (on high copy plasmid);

- I763007 (on low copy plasmid);

- I763033;

- I763015;

- I763012;

- I763005;

- I763031.

10/18/07

• Miniprep:

-I763029;

-I763039(high copy).

• Digestion for:

-I763029 (E/P)

• Band extraction of:

-I763029 (E/P)

• Ligation for:

-pSB4A3 (E/P)

-I763029 (E/P)

• Ligation for:

-I763004 (X/P)

-I763026 (S/P)

• Control Digestion for results impagination:

- I763016 (low copy plasmid);

- I763016 (high copy plasmid);

- I763036;

- I763019;

- I763032;

- I763025 (low copy plasmid);

- I763025 (high copy plasmid);

- I763026;

- I763035.

10/19/07

• Transformation:

-I763040 (on high copy plasmid);::

-I763039;

-I763029 on pSB4A3 (low copy plasmid).

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