From 2007.igem.org

Revision as of 09:20, 23 October 2007 (view source) Silvia.tamarri2 (Talk | contribs) ← Older edit		Revision as of 09:23, 23 October 2007 (view source) Silvia.tamarri2 (Talk | contribs) Newer edit →
Line 40:		Line 40:
	::'''10/18/07'''		::'''10/18/07'''
	*Miniprep:		*Miniprep:
-	::-I763029;	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029];
-	::-I763039(high copy).	+	::-[http://partsregistry.org/Part:BBa_I763039 I763039](high copy).
	*Digestion for:		*Digestion for:
-	::-I763029 (E/P)	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
	*Band extraction of:		*Band extraction of:
-	::-I763029 (E/P)	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
	*Ligation for:		*Ligation for:
Line 54:		Line 54:
	*Ligation for:		*Ligation for:
-	::-I763004 (X/P)	+	::-[http://partsregistry.org/Part:BBa_I763004 I763004] (X/P)
-	::-I763026 (S/P)	+	::-[http://partsregistry.org/Part:BBa_I763026 I763026] (S/P)
	*Control Digestion for results impagination:		*Control Digestion for results impagination:
-	::- I763016 (low copy plasmid);	+	::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-	::- I763016 (high copy plasmid);	+	::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
-	::- I763036;	+	::- [http://partsregistry.org/Part:BBa_I763036 I763036];
	::- [http://partsregistry.org/Part:BBa_I763019 I763019];		::- [http://partsregistry.org/Part:BBa_I763019 I763019];
-	::- I763032;	+	::- [http://partsregistry.org/Part:BBa_I763032 I763032];
-	::- I763025 (low copy plasmid);	+	::- [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
-	::- I763025 (high copy plasmid);	+	::- [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
-	::- I763026;	+	::- [http://partsregistry.org/Part:BBa_I763026 I763026];
-	::- I763035.	+	::- [http://partsregistry.org/Part:BBa_I763035 I763035].

---

Revision as of 09:23, 23 October 2007

10/15/07

• O/N ligations for:

-I763019;

-I763039.

10/16/07

• Transformation of I763019 and I763039.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-I763029;

-I763039 (high copy).

• Control Digestion for results impagination (X/P):

- I763020;

- I763004;

- I763011;

- I763007 (on high copy plasmid);

- I763007 (on low copy plasmid);

- I763033;

- I763015;

- I763012;

- I763005;

- I763031.

10/18/07

• Miniprep:

-I763029;

-I763039(high copy).

• Digestion for:

-I763029 (E/P)

• Band extraction of:

-I763029 (E/P)

• Ligation for:

-pSB4A3 (E/P)

-I763029 (E/P)

• Ligation for:

-I763004 (X/P)

-I763026 (S/P)

• Control Digestion for results impagination:

- I763016 (low copy plasmid);

- I763016 (high copy plasmid);

- I763036;

- I763019;

- I763032;

- I763025 (low copy plasmid);

- I763025 (high copy plasmid);

- I763026;

- I763035.

10/19/07

• Transformation:

-I763040 (on high copy plasmid);::

-I763039;

-I763029 on pSB4A3 (low copy plasmid).

Back