Week 16
From 2007.igem.org
(Difference between revisions)
Line 23: | Line 23: | ||
*We inoculate: | *We inoculate: | ||
::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ||
- | ::-[http://partsregistry.org/Part:BBa_I763039 I763039](high copy). | + | ::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy). |
*Control Digestion for results impagination (X/P): | *Control Digestion for results impagination (X/P): | ||
::-[http://partsregistry.org/Part:BBa_I763020 I763020]; | ::-[http://partsregistry.org/Part:BBa_I763020 I763020]; |
Revision as of 09:26, 23 October 2007
- 10/15/07
- O/N ligations for:
- 10/16/07
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- We inoculate:
- Control Digestion for results impagination (X/P):
- 10/18/07
- Miniprep:
- Digestion for:
- -I763029 (E/P)
- Band extraction of:
- -I763029 (E/P)
- Ligation for:
- Ligation for:
- Control Digestion for results impagination:
- 10/19/07
- Transformation: