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Line 24:		Line 24:
	::-[http://partsregistry.org/Part:BBa_I763029 I763029];		::-[http://partsregistry.org/Part:BBa_I763029 I763029];
	::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).		::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
-	*Control Digestion for results impagination (X/P):	+	*Control Digestion for results impagination (Xba/Pst1):
	::-[http://partsregistry.org/Part:BBa_I763020 I763020];		::-[http://partsregistry.org/Part:BBa_I763020 I763020];
	::-[http://partsregistry.org/Part:BBa_I763004 I763004];		::-[http://partsregistry.org/Part:BBa_I763004 I763004];
Line 44:		Line 44:
	*Digestion for:		*Digestion for:
-	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
	*Band extraction of:		*Band extraction of:
-	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
	*Ligation for:		*Ligation for:
-	::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (E/P)	+	::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1)
-	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)	+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
	*Ligation for:		*Ligation for:
-	::-[http://partsregistry.org/Part:BBa_I763004 I763004] (X/P)	+	::-[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1)
-	::-[http://partsregistry.org/Part:BBa_I763026 I763026] (S/P)	+	::-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1)
	*Control Digestion for results impagination:		*Control Digestion for results impagination:

---

Revision as of 09:49, 24 October 2007

10/15/07

• O/N ligations for:

-I763019;

-I763039.

10/16/07

• Transformation of I763019 and I763039.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-I763029;

-I763039 (high copy).

• Control Digestion for results impagination (Xba/Pst1):

-I763020;

-I763004;

-I763011;;

-I763007 (on high copy plasmid);

-I763007 (on low copy plasmid);

-I763033;

-I763015;

-I763012;

-I763005;

-I763031.

10/18/07

• Miniprep:

-I763029;

-I763039 (high copy).

• Digestion for:

-I763029 (Eco/Pst1)

• Band extraction of:

-I763029 (Eco/Pst1)

• Ligation for:

-pSB4A3 (Eco/Pst1)

-I763029 (Eco/Pst1)

• Ligation for:

-I763004 (Xba/Pst1)

-I763026 (Spe/Pst1)

• Control Digestion for results impagination:

- I763016 (low copy plasmid);

- I763016 (high copy plasmid);

- I763036;

- I763019;

- I763032;

- I763025 (low copy plasmid);

- I763025 (high copy plasmid);

- I763026;

- I763035.

10/19/07

• Transformation:

-I763040 (on high copy plasmid);::

-I763039;

-I763029 on pSB4A3 (low copy plasmid).

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