Week 16

From 2007.igem.org

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::'''10/15/07'''
::'''10/15/07'''
*O/N ligations for:
*O/N ligations for:
-
::-Plac-CI-LacY-GFP;
+
::-[http://partsregistry.org/Part:BBa_I763019 I763019];
-
::-pTet-LacI-T-Plambda-RFP.
+
::-[http://partsregistry.org/Part:BBa_I763039 I763039].
-
*Run on electrophoresis gel and Plac-CI-LacY-GFP band extraction.
+
::'''10/16/07'''
::'''10/16/07'''
 +
*Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763039 I763039].
 +
*We inoculate:
 +
::-[http://partsregistry.org/Part:BBa_I763005 I763005];
 +
::-[http://partsregistry.org/Part:BBa_I763015 I763015];
 +
::-[http://partsregistry.org/Part:BBa_I763004 I763004].
 +
*Trasformation of [http://partsregistry.org/Part:BBa_I763019 I763019], subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
 +
::'''10/17/07'''
 +
*Miniprep of:
 +
::-[http://partsregistry.org/Part:BBa_I763005 I763005];
 +
::-[http://partsregistry.org/Part:BBa_I763015 I763015];
 +
::-[http://partsregistry.org/Part:BBa_I763004 I763004].
 +
*We inoculate:
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029];
 +
::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
 +
*Control Digestion of the following Minipreps for results impagination:
 +
::-[http://partsregistry.org/Part:BBa_I763020 I763020];
 +
::-[http://partsregistry.org/Part:BBa_I763004 I763004];
 +
::-[http://partsregistry.org/Part:BBa_I763011 I763011];;
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763033 I763033];
 +
::-[http://partsregistry.org/Part:BBa_I763015 I763015];
 +
::-[http://partsregistry.org/Part:BBa_I763012 I763012];
 +
::-[http://partsregistry.org/Part:BBa_I763005 I763005];
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031].
 +
*Gel electrophoresis of the fragments above.
-
::'''10/17/07'''
 
 +
::'''10/18/07'''
 +
*Miniprep:
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029];
 +
::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
 +
*Digestion for:
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
 +
*Band extraction of:
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
-
::'''10/18/07'''
+
*Ligation for:
 +
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1)
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
 +
 
 +
*Ligation for:
 +
::-[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1)
 +
::-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1)
 +
 
 +
*Control Digestion of the following Minipreps for results impagination:
 +
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
 +
::- [http://partsregistry.org/Part:BBa_I763036 I763036];
 +
::- [http://partsregistry.org/Part:BBa_I763019 I763019];
 +
::- [http://partsregistry.org/Part:BBa_I763032 I763032];
 +
::- [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
 +
::- [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
 +
::- [http://partsregistry.org/Part:BBa_I763026 I763026];
 +
::- [http://partsregistry.org/Part:BBa_I763035 I763035].
 +
*Gel electrophoresis of the fragments above.
      
      
::'''10/19/07'''
::'''10/19/07'''
 +
*Transformation:
 +
::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763039 I763039];
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
-
[[Bologna | Back]]
+
[[Bologna#Diary | Back]]

Latest revision as of 15:50, 26 October 2007

10/15/07
  • O/N ligations for:
-I763019;
-I763039.



10/16/07
-I763005;
-I763015;
-I763004.
  • Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.


10/17/07
  • Miniprep of:
-I763005;
-I763015;
-I763004.
  • We inoculate:
-I763029;
-I763039 (high copy).
  • Control Digestion of the following Minipreps for results impagination:
-I763020;
-I763004;
-I763011;;
-I763007 (on high copy plasmid);
-I763007 (on low copy plasmid);
-I763033;
-I763015;
-I763012;
-I763005;
-I763031.
  • Gel electrophoresis of the fragments above.


10/18/07
  • Miniprep:
-I763029;
-I763039 (high copy).
  • Digestion for:
-I763029 (Eco/Pst1)
  • Band extraction of:
-I763029 (Eco/Pst1)
  • Ligation for:
-pSB4A3 (Eco/Pst1)
-I763029 (Eco/Pst1)
  • Ligation for:
-I763004 (Xba/Pst1)
-I763026 (Spe/Pst1)
  • Control Digestion of the following Minipreps for results impagination:
- I763016 (low copy plasmid);
- I763016 (high copy plasmid);
- I763036;
- I763019;
- I763032;
- I763025 (low copy plasmid);
- I763025 (high copy plasmid);
- I763026;
- I763035.
  • Gel electrophoresis of the fragments above.


10/19/07
  • Transformation:
-I763034 (on high copy plasmid);
-I763039;
-I763029 on pSB4A3 (low copy plasmid).



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