Week 16
From 2007.igem.org
(Difference between revisions)
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::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ||
::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ||
- | Gel electrophoresis of the fragments above. | + | *Gel electrophoresis of the fragments above. |
Line 69: | Line 69: | ||
::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ||
- | Gel electrophoresis of the fragments above. | + | *Gel electrophoresis of the fragments above. |
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::'''10/19/07''' | ::'''10/19/07''' | ||
*Transformation: | *Transformation: | ||
- | ::-[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid); |
::-[http://partsregistry.org/Part:BBa_I763039 I763039]; | ::-[http://partsregistry.org/Part:BBa_I763039 I763039]; | ||
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid). | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid). | ||
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- | [[Bologna | Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:50, 26 October 2007
- 10/15/07
- O/N ligations for:
- 10/16/07
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- We inoculate:
- Control Digestion of the following Minipreps for results impagination:
- Gel electrophoresis of the fragments above.
- 10/18/07
- Miniprep:
- Digestion for:
- -I763029 (Eco/Pst1)
- Band extraction of:
- -I763029 (Eco/Pst1)
- Ligation for:
- Ligation for:
- Control Digestion of the following Minipreps for results impagination:
- Gel electrophoresis of the fragments above.
- 10/19/07
- Transformation: