Week 16
From 2007.igem.org
(Difference between revisions)
Line 24: | Line 24: | ||
::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ||
::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy). | ::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy). | ||
- | *Control Digestion for results impagination | + | *Control Digestion of the following Minipreps for results impagination: |
::-[http://partsregistry.org/Part:BBa_I763020 I763020]; | ::-[http://partsregistry.org/Part:BBa_I763020 I763020]; | ||
::-[http://partsregistry.org/Part:BBa_I763004 I763004]; | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]; | ||
Line 35: | Line 35: | ||
::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ||
::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ||
+ | Gel electrophoresis of the fragments above. | ||
Line 57: | Line 58: | ||
::-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1) | ::-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1) | ||
- | *Control Digestion for results impagination: | + | *Control Digestion of the following Minipreps for results impagination: |
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid); | ::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid); | ||
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid); | ::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid); | ||
Line 68: | Line 69: | ||
::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ||
+ | Gel electrophoresis of the fragments above. | ||
Revision as of 14:54, 24 October 2007
- 10/15/07
- O/N ligations for:
- 10/16/07
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- We inoculate:
- Control Digestion of the following Minipreps for results impagination:
Gel electrophoresis of the fragments above.
- 10/18/07
- Miniprep:
- Digestion for:
- -I763029 (Eco/Pst1)
- Band extraction of:
- -I763029 (Eco/Pst1)
- Ligation for:
- Ligation for:
- Control Digestion of the following Minipreps for results impagination:
Gel electrophoresis of the fragments above.
- 10/19/07
- Transformation: