Week 16
From 2007.igem.org
Revision as of 07:42, 18 October 2007 by Francesca.bugane2 (Talk | contribs)
- 10/15/07
- O/N ligations for:
- -Plac-CI-LacY-GFP;
- -pTet-LacI-T-Plambda-RFP.
- Run on electrophoresis gel and Plac-CI-LacY-GFP band extraction.
- 10/16/07
- Transformation of Plac-CI-LacY-GFP and pTet-LacI-T-Plambda-RFP.
- We inoculate:
- -Plac-CI;
- -RBS-LacY;
- -Plac-GFP.
- 10/17/07
- Miniprep of:
- -Plac-CI;
- -RBS-LacY;
- -Plac-GFP.
- We inoculate:
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;
- -pTet-LacI-T-pLambda-rfp (high copy).
éDigestion of
- 10/18/07
- 10/19/07
