Week 16
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Revision as of 08:35, 23 October 2007 by Francesca.bugane2 (Talk | contribs)
- 10/15/07
- O/N ligations for:
- -I763019;
- -pTet-LacI-T-Plambda-RFP.
- 10/16/07
- Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
- We inoculate:
- -I763005;
- -I763015;
- -I763004.
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- -I763005;
- -I763015;
- -I763004.
- We inoculate:
- -Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;
- -pTet-LacI-T-pLambda-rfp (high copy).
- Control Digestion for results impagination (X/P):
- - I763020;
- - I763004;
- - I763011;
- - I763007 (on high copy plasmid);
- - I763007 (on low copy plasmid);
- - I763033;
- - I763015;
- - I763012;
- - I763005;
- - I763031.
- 10/18/07
- 10/19/07
