Week 16
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- 10/15/07
- O/N ligations for:
- 10/16/07
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- We inoculate:
- Control Digestion of the following Minipreps for results impagination:
- Gel electrophoresis of the fragments above.
- 10/18/07
- Miniprep:
- Digestion for:
- -I763029 (Eco/Pst1)
- Band extraction of:
- -I763029 (Eco/Pst1)
- Ligation for:
- Ligation for:
- Control Digestion of the following Minipreps for results impagination:
- Gel electrophoresis of the fragments above.
- 10/19/07
- Transformation: