Week 17

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
::'''10/22/07'''
::'''10/22/07'''
*Ligation:
*Ligation:
-
::-[http://partsregistry.org/Part:BBa_I763032 I763032] on [http://partsregistry.org/Part:pSB4A3 pSB4A3];
+
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
*Transformation:
*Transformation:
-
::-[http://partsregistry.org/Part:BBa_I763032 I763032] on [http://partsregistry.org/Part:pSB4A3 pSB4A3];
+
::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
*We inoculate:
*We inoculate:
Line 16: Line 16:
::'''10/23/07'''
::'''10/23/07'''
 +
*Miniprep:
 +
::-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029](high copy plasmid).
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029](low copy plasmid).
 +
 +
*Control Digestion for results impagination:
 +
::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763036 I763036];
 +
::-[http://partsregistry.org/Part:BBa_I763019 I763019];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032];
 +
::-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763027 I763027];
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);
 +
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
 +
 +
*Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032](on pSB4A3).

Revision as of 10:19, 23 October 2007

10/22/07
  • Ligation:
-I763032 (on pSB4A3);
  • Transformation:
-I763032 (on pSB4A3);
  • We inoculate:
-I763040 (on high copy plasmid);
-I763031 (on low copy plasmid), 3 different inocules using 3 different colonies;
-I763029 on pSB4A3 (high copy plasmid).
-I763029 on pSB4A3 (low copy plasmid).



10/23/07
  • Miniprep:
-I763040 (on high copy plasmid);
-I763029(high copy plasmid).
-I763029(low copy plasmid).
  • Control Digestion for results impagination:
-I763016 (low copy plasmid);
-I763036;
-I763019;
-I763032;
-I763025 (low copy plasmid);
-I763027;
-I763029 (low copy plasmid);
-I763029 (high copy plasmid).



10/24/07



10/25/07



10/26/07



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