Week 17
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
::'''10/22/07''' | ::'''10/22/07''' | ||
*Ligation: | *Ligation: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763032 I763032] on [http://partsregistry.org/Part:pSB4A3 pSB4A3]; | + | ::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]); |
*Transformation: | *Transformation: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763032 I763032] on [http://partsregistry.org/Part:pSB4A3 pSB4A3]; | + | ::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]); |
*We inoculate: | *We inoculate: | ||
| Line 16: | Line 16: | ||
::'''10/23/07''' | ::'''10/23/07''' | ||
| + | *Miniprep: | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid); | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029](high copy plasmid). | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029](low copy plasmid). | ||
| + | |||
| + | *Control Digestion for results impagination: | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid); | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763036 I763036]; | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763019 I763019]; | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763032 I763032]; | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid); | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763027 I763027]; | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid); | ||
| + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid). | ||
| + | |||
| + | *Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032](on pSB4A3). | ||
Revision as of 10:19, 23 October 2007
- 10/22/07
- Ligation:
- -[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
- Transformation:
- -[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
- We inoculate:
- -[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
- -[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
- -[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
- 10/23/07
- Miniprep:
- -[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763029 I763029](high copy plasmid).
- -[http://partsregistry.org/Part:BBa_I763029 I763029](low copy plasmid).
- Control Digestion for results impagination:
- -[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763036 I763036];
- -[http://partsregistry.org/Part:BBa_I763019 I763019];
- -[http://partsregistry.org/Part:BBa_I763032 I763032];
- -[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763027 I763027];
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
- Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032](on pSB4A3).
- 10/24/07
- 10/25/07
- 10/26/07
