Week 17

From 2007.igem.org

(Difference between revisions)
Line 7: Line 7:
*We inoculate:
*We inoculate:
-
::-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
+
::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
::-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
Line 17: Line 17:
::'''10/23/07'''
::'''10/23/07'''
*Miniprep:
*Miniprep:
-
::-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
+
::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid).
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid).

Revision as of 12:44, 25 October 2007

10/22/07
  • Ligation:
-I763032 (on pSB4A3);
  • Transformation:
-I763032 (on pSB4A3);
  • We inoculate:
-I763034 (on high copy plasmid);
-I763031 (on low copy plasmid), 3 different inocules using 3 different colonies;
-I763029 on pSB4A3 (high copy plasmid).
-I763029 on pSB4A3 (low copy plasmid).



10/23/07
  • Miniprep:
-I763034 (on high copy plasmid);
-I763029 (high copy plasmid).
-I763029 (low copy plasmid).
  • Control Digestion for results impagination:
-I763016 (low copy plasmid);
-I763036;
-I763019;
-I763032;
-I763025 (low copy plasmid);
-I763027;
-I763029 (low copy plasmid);
-I763029 (high copy plasmid).



10/24/07



10/25/07



10/26/07



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