From 2007.igem.org

Revision as of 14:28, 23 August 2007 (view source) Alice.pasini (Talk | contribs) ← Older edit		Latest revision as of 15:50, 26 October 2007 (view source) Francesca.bugane2 (Talk | contribs)
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-	::'''10/15/07'''	+	::'''10/22/07'''
		+	*Ligation:
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
		+	*Transformation:
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
		+	*We inoculate:
		+	::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
		+	::-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
		+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
		+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
-	::'''10/16/07'''
		+	::'''10/23/07'''
		+	*Miniprep:
		+	::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
		+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
		+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid).
		+	*Control Digestion for results impagination:
		+	::-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
		+	::-[http://partsregistry.org/Part:BBa_I763036 I763036];
		+	::-[http://partsregistry.org/Part:BBa_I763019 I763019];
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032];
		+	::-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
		+	::-[http://partsregistry.org/Part:BBa_I763027 I763027];
		+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);
		+	::-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
-	::'''10/17/07'''	+	*Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]).
-	::'''10/18/07'''	+	::'''10/24/07'''
		+	Data elaboration and wiki updating
-		+	::'''10/25/07'''
-	::'''10/19/07'''	+
		+	Data elaboration and wiki updating
		+
		+
		+	::'''10/26/07'''
		+	Data elaboration and wiki updating
-	[[Bologna | Back]]	+	[[Bologna#Diary | Back]]

---

Latest revision as of 15:50, 26 October 2007

10/22/07

• Ligation:

-I763032 (on pSB4A3);

• Transformation:

-I763032 (on pSB4A3);

• We inoculate:

-I763034 (on high copy plasmid);

-I763031 (on low copy plasmid), 3 different inocules using 3 different colonies;

-I763029 on pSB4A3 (high copy plasmid).

-I763029 on pSB4A3 (low copy plasmid).

10/23/07

• Miniprep:

-I763034 (on high copy plasmid);

-I763029 (high copy plasmid).

-I763029 (low copy plasmid).

• Control Digestion for results impagination:

-I763016 (low copy plasmid);

-I763036;

-I763019;

-I763032;

-I763025 (low copy plasmid);

-I763027;

-I763029 (low copy plasmid);

-I763029 (high copy plasmid).

• Inoculate I763032 (on pSB4A3).

10/24/07

Data elaboration and wiki updating

10/25/07

Data elaboration and wiki updating

10/26/07

Data elaboration and wiki updating

Back